A STUDY OF THE NATURE AND SPECIFICITY OF THE FEULGEN REACTION

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Lessler, Milton A 1915” A study of the nature and specific ity 0f the Feulgen reactionc New York, 19^-9° ' 88 typewritten leaveso illuso, tables,diagrso 29cm0 Thesis (Ph however, made it clear that the

staining with Choudhjari*s colored complex is essentially different from the feulgen reaction in that both chromosomes and cytoplasm become stained. Stedman and Stedman (19^7) were correct in pointing out that the reaction between free hydrolyzed IMA. and the feulgen reagent produces a soluble colored product.

They failed, however, to consider

the fact that, after acid hydrolysis of tissue, the material is washed and this removes any soluble products of hydrolysis before exposure of the material to the feulgen reagent.

The unmasked aldehydes of the

UTA-nucleoprotein which remain fixed in position in the nucleus, subsequently condense with the feulgen reagent to produce an insoluble colored complex which thus must remain at the original site of the IMA in the nucleus. The data presented in Procedure VIZ support this interpretation. It was shown that IMA is strongly bound to protein, is not appreciably liberated by mild acid hydrolysis, nor does it tend to diffuse through gelatin-HTA preparations.

Also, the colored product of the reaction

between hydrolyzed W A ,and the feulgen reagent did not diffuse through

nor was it removed from gelatin-EHA preparations "by repeated washings. Thus, the Stadmans' interpretation of the Feulgen reaction cannot he accepted. Stowell (19^5_: 456-461.

Purification and properties of desoxyribonuclease

isolated from beef pancreas.

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Nature

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Sur la composition prot£ique de chromosomes et

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_________

1947

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John Wiley and Sons, N.I.

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of Feulgen and Dische nucleal reactions.

Chemistry

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Stedman, E.

1944 Jour.

The chemistry of cell nuclei.

Edinborough Med.

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Stedman, E. and E. Stedman

1943a

ent of chromosomes.

Chromosomin - a protein constitu­

Nature

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1943b

Critic of Callan’s article.

1943c

Probable function of histone as a regulator of

mitosis.

Nature

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Nature

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Stedman, E. and E. Stedman

1944-

Chromosomin and nucleic acids

(after Caspersson’s critic)

_________

1947a

Nature

153: 500-502.

The chemical nature and functions of the

components of cell nuclei.

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12:

224-236.

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The function of desoxyribose nucleic acid in

the cell nucleus.

Symp. Soc. Exp. Biol.

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Acid: 231-251.

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The cytological interpretation of the Feulgen

reaction.

Stowell, R.E.

1942

Biochem. Jour.

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Photometric histochemical determination of

thymonucleic acid in experimental epidermal carcinogenesis. Jour. Nat. Cancer Inst.

_________ 1945a

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Effects of Roentgen radiation on the Thymonucleic

acid content of transplantable mammary carcinomas. Research

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1945b

Cancer

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Photometric histochemical determination of

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—86—

Stowell, R.E.

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Feulgen reaction for thjnnonucleic acid.

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Nucleic acids in human tumors. Cancer Research

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The specificity of the Feulgen reaction for

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1947

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Histochemical observations on nucleic acids in

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A spectrophotometric analysis

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•

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Thorell, B.

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A supersensitive Schiff’s aldehyde reagent.

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Verne, J.

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1943

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some properties of sodium thymonucleic acid solutions. Jour. Am. Chem. Soc.

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•

Uber die Verwendbarkeit der Schiffschen fuchsin-

Schwefligsaurereaktion zur quantitative Bestimmung von Thymonucleinsaure.

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Die fuchsinschweflige Saure und ihre

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1932

Comparison between

ultra violet microscopy and Feulgen staining of certain cells.

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