Schistosoma mansoni: Methods and Protocols [1st ed.] 9781071606346, 9781071606353

This volume details protocols for studying Schistosoma mansoni. Chapters guide readers through reviews on current drugs

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English Pages XI, 265 [270] Year 2020

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Schistosoma mansoni: Methods and Protocols [1st ed.]
 9781071606346, 9781071606353

Table of contents :
Front Matter ....Pages i-xi
Praziquantel: An Enigmatic, Yet Effective, Drug (David J. Timson)....Pages 1-8
Computer-Aided Drug Design for the Identification of Novel Antischistosomal Compounds (Jéssika de Oliveira Viana, Marcus T. Scotti, Luciana Scotti)....Pages 9-26
Immunofluorescent Localization of Proteins in Schistosoma mansoni (Rashika El Ridi, Hatem Tallima)....Pages 27-41
Sensitive Fluorescence In Situ Hybridization on Semithin Sections of Adult Schistosoma mansoni Using DIG-Labeled RNA Probes (Lenka Ulrychová, Martin Horn, Jan Dvořák)....Pages 43-53
Collection of Excretory/Secretory Products from Individual Developmental Stages of the Blood Fluke Schistosoma mansoni (Adrian Leontovyč, Lenka Ulrychová, Martin Horn, Jan Dvořák)....Pages 55-63
Labeling of the Schistosoma mansoni Tegument (George Wendt, James J. Collins)....Pages 65-74
A Procedure for Analyzing the Proteomic Proteomics Profile of Schistosoma mansoni Cercariae (Dilhan Perera, Makan Golizeh, Momar Ndao)....Pages 75-84
Proteomic Analysis of Schistosoma mansoni Tegumental Proteins (Mark S. Pearson, Alex Loukas, Javier Sotillo)....Pages 85-92
Measuring Histone Modifications in the Human Parasite Schistosoma mansoni (Ronaldo de Carvalho Augusto, David Roquis, Marion AL Picard, Cristian Chaparro, Celine Cosseau, Christoph Grunau)....Pages 93-107
Step-by-Step Bioinformatics Analysis of Schistosoma mansoni Long Non-coding RNA Sequences (Lucas F. Maciel, Sergio Verjovski-Almeida)....Pages 109-133
Identification of Transposable Elements in Schistosoma mansoni (Gisele S. Philippsen, Ricardo DeMarco)....Pages 135-144
Structural and Functional Characterization of Schistosoma mansoni Cathepsin B1 (Adéla Jílková, Martin Horn, Michael Mareš)....Pages 145-158
Characterization of Schistosoma mansoni Dihydrofolate Reductase (DHFR) (Vitor Hugo B. Serrão, Jéssica F. Scortecci, Humberto D’Muniz Pereira)....Pages 159-172
Characterization of Schistosoma mansoni Glutamate-Gated Chloride Channels (Timothy Lynagh)....Pages 173-183
A Modified ELISA Method to Evaluate the Interaction of Schistosoma mansoni Proteins with Plasminogen (Luis G. V. Fernandes, Rafaela S. Fernandes, Ana L. T. O. Nascimento, Luciana C. C. Leite)....Pages 185-195
Biophysical Analysis of Schistosoma mansoni Septins (Ana Eliza Zeraik, Marina Gabriel Fontes, Ricardo DeMarco)....Pages 197-210
Crystallographic Studies of Triosephosphate Isomerase from Schistosoma mansoni (Pedro Jimenez-Sandoval, Eduardo Castro-Torres, Corina Diaz-Quezada, Luis G. Brieba)....Pages 211-218
Luminescence-Based, Low- and Medium-Throughput Assays for Drug Screening in Schistosoma mansoni Larval Stage (Alessandra Guidi, Roberto Gimmelli, Alberto Bresciani, Giovina Ruberti)....Pages 219-227
Testing of Schistosoma mansoni Vaccine Targets (Weidong Zhang, Adebayo Molehin, Parth Patel, Eunjee Kim, Adam Peña, Afzal A. Siddiqui)....Pages 229-262
Correction to: Measuring Histone Modifications in the Human Parasite Schistosoma mansoni (Ronaldo de Carvalho Augusto, David Roquis, Marion AL Picard, Cristian Chaparro, Celine Cosseau, Christoph Grunau)....Pages C1-C1
Back Matter ....Pages 263-265

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