Guide to Growing Wood Loving Mushrooms for Personal Use

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Guide to Growing Wood Loving Mushrooms for Personal Use

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DON’T OPEN YOUR SPORES BEFORE YOU ARE READY. THEY’RE IN THE FOIL. IF YOU OPEN THE FOIL BEFORE YOU ARE READY, THEN THE SPORES COULD BE CONTAMINATED. DO ALL OF THIS IN A ROOM WITHOUT MOVING AIR. 1. (This is an optional step for those working with spores. No need to do this if you are only working with living liquid cultures.) Build still air box. https://www.shroomery.org/forums/showflat.php/Number/23990888#23990888​ I personally use a box that was for sandblasting. It has doors that open on the side, a window to look in, and to ports for your arms. I removed the gloves from my box, and I would not recommend building your still air box with attached gloves. I cut squares from bandanas and screwed them into place. I cut an opening in them to create a curtain that allows me to stick my hands through. I never did clean my still air box thoroughly. I mostly covered a piece of cardboard with aluminum foil to create a place to set my equipment that is easy to clean. 2. (This is an optional step for those working with spores. No need to do this if you are only working with living liquid cultures.) I take a small flask (mines around 50ml ) or small glass jar and fill it with about 50 ml of water. Take a piece of aluminum foil and seal the flask with it. I usually lay it over the flask, fold the sides of the foil down the flask, then scrunch up the foil to create a lid. I would recommend preparing multiple flasks this way incase you have a fairly large print. There are lots of prints that could easily make multiple flasks worth of spores. It’s also good to do at least two flasks with your first spore print, just in case one of the flasks is contaminated. Cook the flasks in your pressure cooker for approximately 20 minutes. I use the timer on my electric pressure cooker, which I set to high. Let these flasks complete cool before putting spores in them. I like to let them cool down in my still air box to help keep them clean. 3. For live cultures i prepare pint jars with 200ml water, 5.3g dextrose, 2.7g marmite yeast extract, a magnetic spinner and these live culture autoclavable lids of amazon. 6 Pack Liquid Culture... https://www.amazon.com/dp/B074MB7L5Y?ref=ppx_pop_mob_ap_share​. I put these in

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my pressure cooker on high for about 30 minutes. Make sure the lids aren’t screwed on tight. As soon as I take these out, I put a piece of tape on the little hole that’s open. 4. Buy 12 ml syringes that have tips that are not poky. It’s horrible dealing with syringe tips meant to pierce the skin. I bought a hundred pack of syringes that come with tips and screw caps, and sterilization pouches so I can reuse the syringes. I usually prepare about twelve syringes at once. This is because that’s what fits best in my pressure cooker. Place one syringe with one screw cap into the sanitization pouch and seal. I put four of these filled pouches into a pint wide mouth ball jar. I fill three jars in my pressure cooker. I set the timer for 20 minutes. It’s best not to leave these guys in the pressure cooker too long. Once they are done, it is best to move them to a box that allows them to continue to stand up right. If you let them dry lying down on a surface, then it’s possible for contaminates to soak through the wet paper. I have a bunch of syringes sterilized ahead of time, waiting to be used. As for the tips, I store those in a small glass jar full of 70% rubbing alcohol. 5. (This is an optional step for those working with spores. No need to do this if you are only working with living liquid cultures.) Prepare your still air box for transferring the spores from your foil to your flasks. You will need a small glass container of rubbing alcohol (the one full of syringe tips), a small spray bottle of rubbing alcohol (only meant for still air box), metal scalpel (I prefer full metal one with tips that are exchangeable), a candle or small hand held torch, your sterilized flasks and syringes and gloves. I reuse my gloves until they fall apart. I get nitrile gloves that are non-sterile. I am constantly washing my gloved hands with 70% alcohol and wiping them clean with a clean bandana. I keep a small bottle of 70% rubbing alcohol just for washing my hands. I also always where a face mask, which I also reuse. Clean everything in your still air box that you will touch during this process, except the syringes still in their pouches, with 70% rubbing alcohol and wipe it clean. I keep the pointed part of the scalpel in the rubbing alcohol until I’m ready to scrape the spores. Then I take the scalpel out and run the pointy part through the candle or blast it with the torch. 6. (This is an optional step for those working with spores. No need to do this if you are only working with living liquid cultures.) Inside the still air box, take the foil off of the flask(s). Unfold the foil containing your spores. Fold back one side of the foil so that the edge of the foil is as close to the spores as possible. Reducing the distance which the spores must travel over will decrease the chances of contamination. I taco the foil and begin scraping spores into the flask of sterilized water. Depending on the size of the spores you can do multiple flasks. The spores are microscopic. The water doesn’t need to Page 2

become dark with spores, nor do you really need to see any evidence of spores in the water. Once all the spores have been scraped, open a fresh syringe, attach one of the tips that’s sitting in the rubbing alcohol, and run the tip through a flame to completely sterilize. Give a little stir of the water if you would like with your freshly sterilized tip. Then suck up some water. Remove the tip, placing the tip back in the rubbing alcohol, and closing the syringe with the screw cap. Get a new tip for each new syringe and run it through the flame before sucking up more water. You ought to get about 4 syringes worth from each flask. Label the syringes. You could also take a swab for later use at this time as well. See last page on how to prepare swabs. 7. This next part does not require gloves or a face mask. Prepare the growing medium. I take 2 quarts of oak wood pellets for stoves and ovens, and I mix it with ¼ cup of gypsum, ¼ cup calcium carbonate and a quart of water. I let this sit overnight. I cover it with plastic wrap. I mix it once more real good before bed. Then take 2 parts of this wood mixture, 1 part organic brown rice flower and enough water to make it moist. Mix these all together in a bowl. The mixture should be moist and wet. The mixture shouldn’t pool water. Then scoop this into wide mouth pint ball jars. There are two different ways to fill the jars. It all depends on how you want to fruit. If you want to fruit in the jars (perhaps because of temperature), then fill the jars about up with a knuckles worth of medium. Think about making a little patty in the jar. You’ll want it thin enough that you will be able to turn it on it’s side with room to grow fruit on both long sides without being too cramped by the jar. Make sure to wipe of any medium sticking to the sides or top of the jar by itself. These random pieces become prime territory for contaminants. If you are going to fruit outside the jar, then fill the jar almost completely, leaving a little bit of room at the top (this is how I do it for lion’s mane). Cover the top of your jars with a piece of aluminum foil like we did with the flasks. This time take a second square of foil and place it on top of the jar and fold the sides down over the jar. This leaves you with a solid lid that won’t come off easily and a second lid that is easily removable and replaceable. This also provides us with a surface that remains sterile. If you filled up your syringes to 12ml, then I would prepare 12 jars of medium. Place your jars with patties in the pressure cooker for 30 minutes on high. Place your almost full jars in the pressure cooker for 45-60 minutes on high. Remove and let cool. 8. Gloves and a face mask back on, but no need for the still air box, unless you are in a room that has moving air, such as A/C or heating. Have your jar of syringe tips and torch ready. Remove the twist cap from the syringe, and attach a syringe tip. Run this tip through a Page 3

flame to sterilize. Gently remove the top piece of foil from one of the jars, so that you can reuse it. Insert the syringe tip through the remaining piece of foil and inject 1ml of water. Replace the piece of foil that you had removed. Run the needle tip through the flame and repeat the process for all jars. If you have a syringe that contains liquid culture, then you can use this opportunity to inoculate the jars for live cultures that we had prepared earlier. You don’t need to squirt much in from the syringe. You could also use this time to create agar plates and or slants. 9. Build a home for your inoculated jars. I use a fish tank that I lined and topped with styrofoam for insulation. You want this space to remain dark. I place a seed starting mat in the tank to warm the tank to about 86 degrees Fahrenheit. I use the heating mat with the thermometer. It’s ok to pack this tank with jars. Not all the jars need to be on the mat. The jars will be ready when all the substrate has turned white or mostly white. My lion’s mane will start to grow out from underneath the foil if left long enough. 10. Build a fruiting chamber for your jars. Once again I use a fish tank. I use 5 or so pvc pipes that have been cut to about 4” to hold up the tray that comes with an underground filtration system. These trays fit your tank perfectly and are permeable. If you need to heat your mushrooms, then I use the jars with patties, and I place a fish tank heater set to 86 degrees and a fish tank aerator under the tray. Before I fill the tank, I clean it, and everything going into it except the jars. You want to wear your gloves, which you wash all the time with alcohol, and mask during this time. I rinse them off with water. I spray them down with white vinegar, and I leave them to sit for five minutes. Wipe or rinse clean. Then I spray again with hydrogen peroxide. Leave for five minutes and wipe or rinse. I put a dash of hot tub chlorine in the tank before filling it up to about an inch above the tray. Once you place all your jars in the tank, the water should come up the side of the jar by an inch. This contact with the water helps create a warm environment inside the jar. Cover most of the tank with plastic wrap. I leave a gap on one side of the tank for air to come out. I cover this gap with a bandana so that no germs can fall in. I cover the entire tank with a styrofoam top. If you do not need to heat your mycelium, then you can use the jars that are almost completely full. These tanks don’t require the fish tank heater, or added water. I still like to put a dash of chlorine powder in there to help prevent growth at the bottom of the fish tank of various things. These Page 4

tanks end up looking like the following. 11. While the jars are being colonized, you can prepare sterilized water for them. I take four quart jars, because that’s what fits in my pressure cooker, and I fill them with water. I cover them with the two lids of aluminum foil just like the other jars. One foil lid that prevents leaking and another to cover the first to keep things sterile. I sterilize these four jars and their water for about 20 minutes in the pressure cooker. 12. Once the substrate in the jars is white, then it’s time for the fruiting chamber. Get one jar ready at a time to prevent contamination. Where your gloves and use your alcohol to wash your hands and a clean bandana to dry them off. Be sure to wear your face mask too. Remove the foil from a jar. Pour the sterilized water into the jar, enough to cover the mycelium. It may float, that’s ok. Cover the jar with a 2x2 inch sterilized gauze pad. Use a rubber band to secure the top. This prevents infections from spreading like wildfire throughout your colonies. Very cool. Very swag. Place the jar in the heated water of the fish tank. Come back the next day with your mask and gloves. One at a time, remove the water from the jars. I keep the scalpel tip in the rubbing alcohol jar with the syringe tips during this time. When I go to pour out the water, I use the sterile scalpel tip to hold back the mycelium. I then arrange the mycelium so that the disk is on its side standing up in the jar. This allows for maximum surface area for the fruit to grow. Secure the gauze pad back on top. Wait for mushrooms to appear! If it looks like the gauze will mess with your fruit, then I pop the rubber band off the jar. 13. I find the mushrooms I deal with take about two weeks to appear and another week to finish fruiting. When it’s time to harvest, I use my gloves and mask. For jars with patties, I pick the fruit, then cover the mycelium with sterilized water again. The fruit is dried. The mycelium is left overnight to soak up water. I use the scalpel to hold back the mycelium when I pour the water out the next day. For mycelium that fruited on the trays, Page 5

I pick the fruit, and then lay the mycelium on it’s side, and pour sterilized water on them. I let them hang out overnight. Then in the morning I tilt the trays so the water pours into the bottom of the tank. I set the mycelium backup right, and wait for more fruit or for the cake to die. 14. Prepare your still air box for making prints. Inside the box you will need two squares of tin foil, a tiny little dropper of sterilized water, 2 glass jars and some forceps. Clean the dropper and the jars with at least the rubbing alcohol and wipe clean with a clean bandana. I use a little alcohol wipe to clean the pieces of foil. 15. Once you see a nice cap that has opened all the way, then you can pick it. Use your gloves and mask. I have the scalpel ready, but this time I don’t let it sit in the alcohol. I dip it in alcohol and then run it through the flame to sterilize it. I have a needle handy. I would clean or sterilize the needle, but it’s not super important. Stick the needle through the cap and into the stem. This gives you a handle. Now cut the stem off of the cap using the sterilized scalpel. Hold onto your cap using the needle so that the gilled side is facing down. Using your hand as a shield from the bandana of your still air box port, bring the cap into the still air box and place it gilled side down on the foil. Remove the needle. Drop a drop of sterilized water on the cap. You can place multiple caps on the same piece of foil so long as there is room. Cover your cap with one of the jars. This will prevent your cap from drying out and not producing spores. Leave the cap until the next day. The next day you will remove the jar and use the forceps to remove the caps from the foil. Fold the foil over your print. Dry the cap. You can now repeat this entire process for more spores and mushrooms. 16. For mushrooms like lion’s mane, where it’s super difficult to catch spores, I use live culture cloning. Before you pick your mushrooms, pick or cut off a small piece of the mushroom. Use clean gloves and a sterile scalpel, or use a syringe to stab a piece of the mushroom. At this piece of mushroom to your pre sterilized liquid culture jar. I like to add the piece through the little hole plugged by the rubber stopper. I have a magnetic stirring and heating unit. I have found this makes the best live liquid culture. I set the jar on heat, give it a whirl and a little heat, about 80 degrees fahrenheit. I do this a couple times a day for a couple days until I see the mixture begin to get cloudy. Then I set up my sterile syringes and and needle tips in the alcohol. I attach the needle to the syringe, fire it with my torch, and then suck up as much culture as I can through the hole for the rubber stoppers. I replaced the needle with the cap, and wala! Done. You can repeat the process. I keep these syringes in my fridge. 17. The stem and any other mushrooms picked can be dried for later use. To do so, place on a dehydrator on the lowest setting. Wait until it’s fairly dry and then place in a container containing silica beads. I use three containers. One big oxo pop top container and two quart jars. I fill them halfway with silica beads, the kind that change color from blue to pink as they collect water. They are reusable. I place paper towels Page 6

over the beads to protect the mushrooms. I use the oxo container for the freshest mushrooms. The next day when I have picked more, I love the mushrooms from the big container to one of the jars. The following day I will move the mushrooms from the big container to the empty jar. On the following day I’ll empty the first jar I filled and use it for the mushrooms coming from the big container. 18. I love making tea with my wood loving friends. Soak for thirty minutes in enough cold water to cover the following herbs; 9g jujube date, 9g fresh ginger, 2 g lion’s mane, 2g wood ear, 2g red reishi, 2g black reishi, 2g maitake, 2g shiitake and 12-24g Syberian ginseng, if you can find it. Bring to boil, and simmer for 45 minutes. Strain the herbs from the liquid. If you were only able to find concentrated powder for herbs such as the Siberian ginseng, then now would be the time to dissolve that powder into the hot liquid. Divide the liquid into 2 parts (one for each day), and then divide those two parts into two or three parts (one for morning, one for evening, and a third one can be for midday if you like). Drink tea warm. Reheat, or add hot water to it. I like to add my mushroom tea serving to my thermos, and then top off with hot water. I sip on this throughout the morning. I like the three doses. It’s recommended to take between meals. This tea helps strengthen your immune system, prevent diseases, give you energy (if you have Siberian ginseng), and balance your nutritive energy with your protective energy. If you are a really warm person, then go light on the ginger. If you are a sticky, phlegmy person, then go light on the jujube date. If you come across a real beauty of a fruit, then you can clone it using the liquid culture, or better yet, using agar tech. If you have difficult mushrooms to collect spores from and want to collect spores or if you want to share part of a spore print then use swabs! all you need to do for swabs is : 1 take your DRY swabs and wrap them tightly in foil : take the foil wrapped swabs and place them into a jar with a lid(Fitted lid or foil lid(You dont want them getting wet at all) : place that jar in the Pressure Cooker for 30 to 45 min : When ready to start your swabbing have your tools ready in this case the swabs and Glovebox...take your PE mushroom head and get an end up inside the cap and give it a slight twiist back and forth till the swab end looks black(Full of spores)...NOTE : Try not to touch the stipe (stem) of the mushroom with the swab at all...I usually cut the cap in half, makes it a little easier...so once you have your spore ridden swab just wrap it back up in the foil for later use Swabs can be used to inoculate agar plates or other sterile mediums. Agar tech https://www.shroomery.org/forums/showflat.php/Number/21922023 https://www.shroomery.org/forums/showflat.php/Number/22721954/vc/1#22721954 https://www.shroomery.org/forums/showflat.php/Number/18182209

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500ml distilled h2o 9-10g Agar 6-8g Extra Light Malt Extract Bring the water to a boil shut off the heat, add dry ingredients, stir until fully dissolved, load media bottle, sterilize 15-20 minutes @ 15-17psi. Pour once cooled to 110-108F. Let plates cool and dry unwrapped infront of flow hood or in still air box. Wrap plates with Saran or Parafilm. Store away from heat sources that will drive water from agar and create condensation. There's a million ways to prepare agar. That's my preferred and creates crystal clear plates.

For wood lovers add sawdust to agar plate For cubes and pans add poo For cubes I use half pint mason jars and I use 500 ml water 10g agar 7 gs dry dog food I bring agar and dry dog food powdered to a boil then bring down to simmer for 3 mins then i pour 60 or so ml per jar i let cool aside so it doesn't build condensation then I tighten my lids add to pc and pc for 60 mins then let agar kool overnight then ur good to go For slants https://www.shroomery.org/forums/showflat.php/Number/18182209 You can find the most up to date version of these instructions on the library generator using the download books instructions.

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