Eymard Poydock PhD: Vitamin C and Vitamin B12 for Cancer

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Eymard Poydock PhD: Vitamin C and Vitamin B12 for Cancer

Eymard Poydock PhD (Vitamin C + Vitamin B12)

November 11, 2008
https://beatis.wordpress.com/2008/11/11/list-of-alternative-cancer-treatments/

Alternative cancer treatments
Filed under: alternative medicine, health fraud, Uncategorized — Tags: alternative medicine, baking soda, cancer, candida albicans, chemo, fungus, health fraud, quackery, sodium bicabonate, tullio simoncini — beatis @ 3:23 pm
Here’s a list coming from a Dutch guy named Ed. He and others think the greater part of the more than 2000 (!) alternative treatments for cancer are BS.

No stand-alone successful alternative therapies for cancer exist or will ever exist. We hope that by describing the present chaos, the

Citation preview

Vitamins Against Cancer Randolph Byrd John Yates Important advances may take place in the most underfunded, understaffed lab. Dedication and inspiration sometimes get results that money can't. The work of Sister Mary Eymard Poydock, Ph.D., professor of biology and director of cancer research at Mercyhurst College is a case in point. The Mercyhurst labs are not large or well endowed. But 20 years of painstaking work by Sister Eymard and her associates have now produced results which indicate that a combination of vitamins C and B ] 2 may have a powerful effect against cancer. While Sister Eymard's work has been confined to laboratory animals, the implications for further research are obvious. For 20 years, Sister Eymard has been testing a substance that had been advanced as a possible anticancer agent. The first results were promising, and the name "Mercytamin" (from the Sisters of Mercy) was applied to the substance. Mercytamin was actually a combination of vitamins C, B12 and a variety of enzymes. Through scientific testing of the solution, the enzymes and other chemical additives were eliminated as possible active agents. Finally only the vitamins remained, and in 1978 the name Mercytamin was dropped. Sister Eymard held back on publishing her findings until she was certain the results she was getting were true. "We've done enough experiments now, testing hundreds of mice, to establish that it works," Sister Eymard says. "We've got it down to a point now where if you do it according to the 'recipe,' it will work every time." Sister Eymard and her research staff implanted three common types of cancers into laboratory mice— sarcomas (cancerous growths of connective tissues), carcinomas (cancers of skin-like tissues), and leukemias (cancers of the blood-forming

organs). Those cancerous tissues were implanted both in the abdomens and under the skin of the mice. The mice were then injected with the mixture of vitamins C and B 12 (in a ratio of one part B 12 to two parts C) near the site of the tumor transplant. Within four days, some of the tumors from the abdomens of the mice were removed, and the cells were examined under the microscope. There was a dramatic change in the tissue. The cancerous cell division had stopped completely. Tumors Didn't Grow The tumors growing under the skin were treated the day after implant with the C/B12 combination. The results were similar—no tumors would grow. In the control animals (those mice with transplanted tumors which were not given the C/B12 combination), the tumors continued to grow at a rapid rate.

A biologist who's also a Sister of Mercy has found that C and B12 can spell doom for malignant cells. Inhibiting tumor growth was only part of the results of Sister Eymard's experiments. She also wanted to see if the C/B]2 mixture would prolong the lives of animals already suffering from cancer. To find out, Sister Eymard and her colleagues injected the mixture into the abdomens of diseased mice near the cancerous growths for seven successive days. Treated animals lived longer than those mice not given C and B 12 . In fact, all the treated mice outlived the control group. It appeared that the combination of C and B12 not only inhibits the growth of cancer cells, but also prolongs the lives of animals impregnated with cancer. "There are few things presently on the market that will insure a 100 percent survival rate with cancer," Sister Eymard says, "but we had a 100 per-

cent survival rate after the controls were dead. Most of the treated mice outlived the controls two or three weeks." To be sure that it was really the C/B12 concoction and not an unknown factor present in mice that was responsible for her results, Sister Eymard conducted experiments on free-living cancer cells growing on a culture medium. The vitamin C/B12 complex was used as a treatment on three types of cancer cells, and on healthy cells as well. The treated cells, the untreated control group and the healthy cells were left to incubate. At the end of the incubation period, the untreated control group was infested with cancer cells. In the treated group, however, not one cancer cell of any of the three types was to be found. The healthy, noncancerous cells were unaffected by the C/B12 complex. It appeared that Sister Eymard had found a cancer-inhibiting agent that not only stops many kinds of cancer, but does so with absolutely no side effects in healthy tissue. Sister Eymard also tested each vitamin separately to determine if either was primarily responsible for the anticancer effects. The combination of the two vitamins, however, always performed much more effectively than either one alone. Tests showed that the combination might be working by boosting the animals' immune systems to fight the cancer. Sister Eymard is confident that with more experimentation, especially on larger mammals and humans, the vitamin C/B12 combination could prove to be a useful preventive weapon in the fight to eliminate the second leading cause of death in America. • Reprinted by special permission of Prevention Magazine/Rodale Press.

Randy Byrd is a 1974 graduate of Mercyhurst College where he majored in environmental studies. He is a free-lance writer and born naturalist. John Yates is a former senior editor of Rodale Press.

way, binds to pathogens and initiates their lysis. C1q is similar

were reduced in ascorbate-treated animals . By 20 weeks, five

to collagen and contains helical regions with hydroxyproline residues . Animals receiving tissue-saturating doses of ascorbate had significantly higher levels of C1q.

times more mice in the control group had developed large lesions than in the group given high doses of ascorbate . In RIII mice, the spontaneous appearance of mammary tumors was significantly delayed in animals treated with ascorbate . The median age at appearance of first tumor was 83 weeks in controls and

In Vitro Effects on Tumor Growth 125 weeks in the group given high doses of ascorbate . The

Investigators also discussed the role of ascorbic acid in tumor cell growth and malignant transformation . Dr Luminita Ibric (Children's Hospital , Los Angeles, Calif) reported that ascorbic

highest dose these animals received was approximately 10 g of vitamin C per kilogram of body weight.

acid has been shown to irreversibly suppress methylcholanthrene-induced transformation of C3H/10t1/2 cells . The effect appears to be related to the redox potential of the cells and

Dr Joachim Liehr (University of Texas Medical Branch, Galveston) showed that ascorbic acid inhibits estrogen-induced renal tumors by 50% in hamsters by reducing the concentration of estrogen quinone metabolites and their DNA adducts . He also

perhaps to changes in lipid metabolism with ascorbate treatment.

described the biochemical mechanism involved in the development of estrogen-induced cancers through nonhormonal actions .

The effect of ascorbate is not limited to chemically induced

Dr Eymard Poydock (Mercyhurst College, Erie , Pa) described

transformation. Dr Richard Schwarz (Lawrence Berkeley Laboratory, Berkeley, Calif) has shown that ascorbate prevents oncogenic transformation of cells infected with the Rous sarcoma virus . In his study, the ascorbate reduced virus production and promoted synthesis of proteins associated with differentia-

12 on implanted the inhibitory effect of ascorbate and vitamin B₁₂ Ehrlich carcinoma and L1210 leukemia in mice . All control

tion. Dr Schwarz was not able to explain the mechanism of this

mice had died by day 19, whereas more than 50% of treated mice were still free of tumors after 60 days. In the presence of vitamin B12, the cobalt nucleus is released and attaches to ascorbic acid, possibly forming cobalt ascorbate . The cobalt-vitamin

inhibitory effect on Rous virus production. The effect of ascorbate on human immunodeficiency virus (HIV) replication was described by Dr Raxit Jariwalla (Linus

C complex prevents mitoses in several transplantable murine tumors without damage to normal cells.

Pauling Institute, Palo Alto, Calif) . In chronically infected T lymphocytes exposed to high but nontoxic levels of sodium as-

reported on the effect of ascorbic acid and its synthetic

Dr Robert Smart (North Carolina State University, Raleigh)

corbate, reverse transcriptase activity was reduced by more than

lipophilic derivative ascorbyl palmitate on phorbol ester-induced skin tumor promotion in CD- 1 mice . Large topical doses

99%. Levels of p24 HIV core antigen in the culture supernatant were reduced by 90%. In acutely infected cells , ascorbate

of ascorbic acid and small doses of ascorbyl palmitate inhibited tumor promotion and ornithine decarboxylase activity. Ascorbyl

reduced syncytium formation by 93% . The continuous presence of ascorbate was necessary for HIV suppression . Ascorbate did not inactivate extracellular virus . Under the same conditions , there were no detectable inhibitory effects of ascorbate on

palmitate also inhibited DNA synthesis. The number of tumors per mouse was reduced by 90%, and the number of mice with tumors was reduced by 86% . Dietary ascorbic acid inhibited the induction of ornithine decarboxylase but did not prevent tumor

growth, metabolic activity, or rate of protein synthesis in uninfected T lymphocytes .

promotion or epidermal DNA synthesis .

Additional studies with results that may have clinical applications were presented by Dr Chan Park (Texas Tech University,

mammary tumor xenografts in mice was described by Dr Constance Tsao (Linus Pauling Institute ) . Ascorbic acid, along with

Lubbock) . Dr Park found that leukemic colony-forming cells from patients with acute myeloid leukemia or the myelodysplastic syndrome can be divided into three groups : one stimulated ,

cupric sulfate, inhibited the growth of tumor fragments implanted beneath the renal capsules of immunocompetent mice. Ascorbate added to the diet had no effect, but, when in-

another suppressed , and the third not affected by ascorbate. Of approximately 500 cases studied , ascorbic acid enhanced colony growth in 35 % and suppressed growth in 15%. These effects

corporated into the drinking water, it was effective . A stereoisomer of ascorbic acid, D- isoascorbic acid, which has

were not seen in normal controls or in patients with chronic myelogenous leukemia. Other redox agents, including vitamin E, were ineffective . D-Ascorbic acid was much less effective

The inhibiting effect of ascorbic acid on the growth of human

only 5% the antiscorbutic potency of L - ascorbic acid, had similar antitumor activity. It appears, therefore, that in this system, the antitumor activity of ascorbic acid is due not to its metabolism as a vitamin but to its chemical properties.

than L-ascorbic acid. These observations had prognostic value . Patients with myelodysplastic syndrome whose colonies were stimulated by ascorbate had shorter survival times than patients whose colonies were not affected .

Adjuvant and Toxicity- Reducing Therapeutic Applications

In Vivo Effects on Tumor Growth

Dr Paul Okunieff (Harvard Medical School, Cambridge , Mass) described the radioprotective effects of ascorbic acid on skin and bone marrow in mice with established fibrosarcomas.

There was considerable interest in the session on tumor growth and vitamin C. The first speaker was Dr Linus Pauling (Linus Pauling Institute) , who reviewed two large studies using animals . Skin tumors in hairless mice exposed to UV radiation

548

In nonirradiated animals, ascorbic acid did not modify or prevent tumor growth. When it was given immediately preceding radiation, there was a significant reduction in the toxic effects of radiation in both skin and bone marrow, but the tumor

Journal of the National Cancer Institute

2/24/24, 10:40 PM

Vitamin B12 turbocharges vitamin C to kill cancer

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Nun’s divine cancer cure… WIPED from history! (https://hsionline.com/2018/11/06/nunsdivine-cancer-cure-wiped-from-history/)

You’ve probably never heard of Sister Mary Eymard Poydock before.   And, trust me… that’s EXACTLY the way that mainstream medicine wants it.   Decades ago, in a tiny, poorly funded laboratory at a Catholic university, Sister Eymard did the impossible.   She developed a natural CURE for cancer.   Tumors stopped growing… and literally DISAPPEARED.   And Sister Eymard wasn’t just a nun – she was also a PhD-educated biology professor, researcher and board member of the https://hsionline.com/2018/11/06/nuns-divine-cancer-cure-wiped-from-history/

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Vitamin B12 turbocharges vitamin C to kill cancer

American Cancer Society.  So why haven’t you heard of her life-saving work?  Because the mainstream medical establishment has spent years ERASING and COVERING UP Sister Eymard’s life’s work.   Here’s the TRUTH about her cancer breakthrough… the truth you were NEVER supposed to know.   Cancer be damned!  When someone devotes to a life of faith, they spend their time serving those in need.   Some take a vow of silence.    Sister Mary Eymard Poydock found a cure for cancer.  A Sister of Mercy, she spent the greater part of the 1970s, 80s, and 90s working at Mercyhurst University in Pennsylvania.   And she painstakingly studied how a combination of vitamins C and B12 could KILL. cancer.  By 1976, she and her research team had gotten to the point where anyone could follow her “recipe.”  In mouse studies, the vitamin combo stopped cancerous cell division completely in the most commonly diagnosed cancers, carcinomas… as well as the deadliest forms of cancer, sarcomas… and leukemias.  And while it put the brakes on tumors that would’ve kept growing on their own without treatment, Sister Eymard’s discovery https://hsionline.com/2018/11/06/nuns-divine-cancer-cure-wiped-from-history/

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Vitamin B12 turbocharges vitamin C to kill cancer

also prolonged lives.  That, of course, is the number-one outcome that any cancer patient looks for.  In follow-up studies, Sister Eymard used vitamins C and B12 together to ERADICATE cancer cells in a lab.   Now, as good as we’ve found intravenous vitamin C to be at wiping out cancer, it doesn’t have a perfect record on its own.  But with cobalt-ascorbate (this combination of vitamins C and B12), this Sister of Mercy claimed she had a 100% success rate.  These aren’t just tall tales of blind faith. Sister Eymard published multiple studies in several of our top peer-reviewed medical journals.   In 1985, her study in the American Journal of Clinical Oncology found that an oxidized form of vitamin C (dehydroascorbic acid) stopped the division of leukemia cells in mice… and that the treated mice survived longer.  And in a 1991 study published in The American Journal of Clinical Nutrition, all untreated mice injected with tumor cells died by day 19… while more than half the treated mice were still alive after 60 days.  That might not seem like a long time — but to a lab mouse, whose average lifespan is only a couple of years, IT IS!  Russian researchers found in 2007 that B12 ramps up vitamin C’s cancer-killing qualities by more rapidly provoking the accumulation of hydrogen peroxide, which is deadly to cancer cells.  https://hsionline.com/2018/11/06/nuns-divine-cancer-cure-wiped-from-history/

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Vitamin B12 turbocharges vitamin C to kill cancer

And a lab study out of Russia found the combination kills cancer cells yet remains non-toxic to healthy cells… and the results were because of the COMBINED action of the two vitamins.    Since then? Our “great minds” of science have dropped the ball.   In fact, your doc may tell you that vitamin C might actually offset vitamin B12 when taken together – but that only applies to oral supplements.  If you want to kill your cancer, you need to bypass your gut and deliver the vitamins directly into the bloodstream via infusions.  Intravenous vitamin C is becoming more widely offered and accepted even by mainstream medical clinics. Ask your cancer care team how to throw some B12 infusions — this divine “nun’s recipe” — into the mix. 

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Cancer Recipe – Cforyourself

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A visitor, Clarence Moury, sent me a letter discussing his and wife’s experience with cancer using the following “recipe” he found in a Rodale Press publication of which he did not mention the name. Here is the “recipe” and please also read his letter.

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CANCER CHAPTER 72

The work of Sister Mary Eymard Poydock, Ph.D., director of cancer research and former professor of biology at Mereyhurst College in Erie, Pennsylvania, is a case in point. The Mercyhurst labs are not large or well endowed. But 20 years of painstaking work by Sister Eymard and her associates have now produced results, which indicate that a combination of vitamins C and B 12 may have a powerful effect against cancer. While Sister Eymard’s work has been confined to laboratory animals, the implications for further research are obvious. For 20 years, Sister Eymard, has been testing a substance that ha been advanced as a possible anti-cancer agent. The first results were promising, and the name “mercytamin” (from the Sisters of Mercy) was applied to the substance. Mercytamin was actually a combination of vitamins C, B12 and a variety enzymes. Through scientific testing of the solution, the enzymes and other chemical additives were eliminated as possible active agents. Finally, only the vitamins remained, and in 1978 the name marcytamin was dropped. Sister Eymard held back on publishing her findings until she was certain the results she was getting were true. We’ve done enough experiments now, testing hundreds of mice, to establish that it works,” Sister Eymard says. “We’ve got it down to a point now where, if you do it according to the ‘recipe,’ it will work every time. ” https://cforyourself.com/cancer-recipe/

Dr. Linus Pauling, the Nobel prizewinning scientist, was a chief advocate of the importance of supplementing our diets with Vitamin C to promote optimum health and cure disease. "Do not let either the medical authorities or 1/3

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Cancer Recipe – Cforyourself

Sister Eymard and her research staff implanted three common types of cancer into the Translate Toggle laboratory mice- sarcomas (cancerous growths of connective tissues), carcinomas (cancers politicians of skin like tissues) and leukemias (cancers of the blood-forming organs). Those cancerous mislead you. tissues were implanted both in the abdomens and under the skin of the mice. The mice Find out were then injected with the mixture of vitamins C and B 12 (in a ratio of one part B12 to two what the parts C) near the site of the tumor transplant. facts are, and make your Within four days, some of the tumors from the abdomens of the mice were removed, and own the cells were examined under the microscope. There was a dramatic change in the tissue. decisions The cancerous cell division had stopped completely. Tumors Didn’t Grow. about how to live a The tumors growing under the skin were treated the day after implant with the C- B 12 happy life combination. The results were similar-no tumors would grow. In the control animals, those and how to mice with transplanted tumors which were not given the C B12 combination), the tumors work for a continued to grow at a rapid rate. better Inhibiting tumor growth was only part of the results of Sister Eymard’s experiments. She world." also wanted to see if the C-Bl2 mixture would prolong the lives of animals already suffering - Linus from cancer. To find out, Sister Eymard and her colleagues injected the mixture near the Pauling cancerous growths of diseased mice for seven successive days. Treated animals lived longer than those mice not given C and B 12. In fact, all the treated mice outlived the control group. It appeared that the combination of C and B12 not only inhibits the growth of cancer cells, but also prolongs the lives of animals impregnated with cancer. “There are few things presently on the market that will ensure a 100 percent survival rate with cancer,” Sister Eymard says, “but we had a 100 percent survival rate after the controls were dead. Most of the treated mice outlived the controls two or three weeks.” To be sure that it was really the C-B 12 concoction, and not an unknown factor present in mice, that was responsible for her results, Sister Eymard conducted experiments on freeliving cancer cells growing on a culture medium. The vitamin C- B12 complex was used as a treatment on three types of cancer cells and on healthy cells, as well. The treated cells, the untreated control group and the healthy cells were left to incubate. At the end of the incubation period, the untreated control group was infested with cancer cells. In the treated group, however, not one cancer cell of any of the three types was to be found. The healthy, noncancerous cells were unaffected by the C-B 12 complex. It appeared that Sister Eymard had found a cancer-inhibiting agent that not only stopped many kinds of cancer, but did so with absolutely no side effects in healthy tissue.

Please visit An Overview of Linus Pauling and his Work & Linus Pauling and the Twentieth Century: An Exhibition & Profiles in Science: The Linus Pauling Papers This site is dedicated to his life and work.

Sister Eymard also tested each vitamin separately to determine if either was primarily responsible for the anti-cancer effects. The combination of the two vitamins, however, always performed much more effectively than either one alone. Tests showed that the combination might be working by boosting the animals’ immune systems to fight the cancer. Sister Eymard is confident that, with more experimentation, especially on larger mammals and humans, the vitamin C-B 1 2 combination could prove to be a useful preventive weapon in the fight to eliminate the second leading cause of death in America. The scientific community has taken note of Sister Eymard’s findings. A spokesman for the American Cancer Society, which helps fund Sister Eymard’s research, told us “The physicians on the committee which reviewed Sister Eymard’s work were most impressed with the results she was getting. She has come forth with work of considerable promise. ” https://cforyourself.com/cancer-recipe/

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Cancer Recipe – Cforyourself

Sister Eymard herself takes a modest stance. “I’m glad you’re looking into this,” She told us. “Every bit of information helps to educate the public. It might give them some hope, and that’s what they need most.”

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Expl Cell Biol. 50: 88-91 (1982)

Influence of Vitamins C and B n on the Survival Rate of Mice Bearing Ascites Tumor M. Eymard Poydock, D. Reikert, J. Rice Mercyhurst College, Erie, Pa., USA

Key Words. Ascites tumors • Vitamin C ■Vitamin B12

Introduction Current animal experimentation and clin­ ical findings suggest a new dimension in can­ cer therapy. The number of publications re­ garding the relationship between vitamins and cancer is steadily increasing. Nutritional deficiencies that often coexist with malig­ nancy in humans also appear to be associated with lowered resistance [9, 10]. Basu et al. [1] and Basu [2] reported that nutrition and ab­ sorption of certain vitamins seem to improve the immunity of the body. A number of workers [8,11] have explored the efficacy of vitamin A treatment alone, and in combina­ tion with anticancer agents. Kaplan and Busford [12] suggested that vitamin B i 2 defi­

ciency impairs the metabolic activity associ­ ated with phagocytosis suggesting that this vitamin is influential in augmenting the host’s natural resistance. Evidence is accu­ mulating that vitamin C may be a determi­ nant factor in host resistance to cancer [4-7, 13, 15], and that large doses directly interfere with the metabolism of neoplastic cells. Benade et al. [3] found that ascorbic acid exhibited cytotoxic activity in Ehrlich carci­ noma cells in tissue culture, while Park et al. [15] reported that ascorbic acid suppressed the growth of leukemic cells in vitro. Previous studies in this laboratory by Poy­ dock et al. [16] demonstrated complete inhi­ bition of mitoses in sarcoma 37. P388 leuke­ mia and Ehrlich carcinoma after treatment

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Abstract. The survival rate of mice bearing P388 leukemia and Ehrlich carcinoma was increased after treatment with a mixture of vitamins C and Bi2. All the mice receiving the vitamins outlived the control group. At the termination of the experiment, 30 days later, 50% of the treated mice appeared normal and healthy, whereas the remainder showed signs of tumor distention.

with a mixture of vitamins C and B^, with no apparent toxic side-effects. More recently the author [ 17] reported that cell division was inhibited in two ascites tumors after injec­ tions of dehydroascorbic acid in mice. It seemed important, therefore, to investigate the effect of the vitamins on the longevity of mice bearing ascites tumors.

Methods and Materials The transplantable P388 leukemia and Ehrlich carcinoma in the ascites form were used in this assay. To carry the ascites tumor in stock mice they were injected (i.p.) with 0.1 ml (106) washed ascites cells harvested on the 7th day after tumor implantation. P388 was maintained in DBA/2 stock mice, whereas Ehrlich carcinoma was propagated in HA/ICR Swiss mice. Two experiments were designed for this study. Experiment 1 utilized P388 leukemia in BDE| mice and experiment 2 tested Ehrlich carcinoma in HA/ICR Swiss mice. A total o f 100 mice (50 lest.

Fig. 1. Survival of mice bearing P388 leukemia after treatment with vitamins C and B |2. 100 mice were sampled; 50 treated (•) and 50 con­ trol (o). + = Terminated.

89

50 control) were used for each experiment. All the ani­ mals were within a 3-gram weight range. They were injected (i.p.) with 0.1 ml (10*) washed ascites cells taken from a stock mouse. 24 h after tumor transplantation the mice in each experiment were divided equally into two groups and designated as test and control. The test mice were inoculated once daily (i.p.) with 6 mg/0.2 ml vitamin C and B 12 mix­ ture for 8 successive days. The control animals re­ ceived an equal volume o f saline. During the weekend the mice received no treatment, then the vitamin injections were continued on alternate days for 3 weeks. The total number o f treatments was 17. The vitamins, in crystalline form, were prepared by introducing 0.01 g B)2, 0.01 g sodium ascorbate (NaASC), and 0.01 g L-ascorbic acid into a small tube. A sufficient number o f tubes with the dry crystals were prepared in advance. Immediately before injection, 1 ml sterile distilled H20 was added to the tube. The solution was withdrawn into a l-ml tuberculin syringe, a Millex-GS filter unit attached, and the needle added. Only the amount needed (1 ml) to inject 5 mice was diluted at one time. Vitamin B,2 (hydroxocobalamin) was received from Merck Chemical Company. The L-ascorbic acid and its salt NaASC, were purchased from Sigma Chemical Co. The Millex-GS filter unit was obtained from Millipore Corp.

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Influence o f Vitamins C and B 12 on Survival

90

Poydock/Reikert/Rice

Results Figures 1 and 2 show the percent survival of mice bearing P388 leukemia and Ehrlich carcinoma, respectively, after treatment with a mixture of vitamins C and B12 - The sur­ vival of mice receiving the vitamin mixture was significantly prolonged when compared with the control mice. The earliest deaths in the vitamin-treated group occurred on the 32nd day after tumor transplantation, whereas none of the control animals survived longer than 22 days. The median survival time for the un­ treated mice, both P388 and Ehrlich, was 15 days. All the mice receiving the vitamins survived at least 30 days after tumor trans­ plantation. Then a few, showing signs of can­ cer growth (distended with tumor tissue), died. After all the control mice and the test mice distended with tumors had died, the experiment was continued for another 30 days, then terminated. At this time about 50% of the animals receiving the vitamin treatment were still alive.

Discussion and Conclusion Treatment with a mixture of vitamins C and B12 increased the survival rate of tumor­ bearing mice. This substantiates reports pub­ lished by Cameron and Campell [4] and Ca­ meron and Pauling [5], where supplemental treatments of ascorbic acid significantly pro­ longed the survival time of patients with ter­ minal cancer. The mechanism of action which brings about the destruction of neoplastic cells is still unclear. The vitamin mixture may alter the cell membrane of malignant cells ena­ bling the T lymphocytes to recognize them as foreign. Then, as Old [14] suggests, T cells kill the tumor cells by releasing a toxic factor that disrupts the cell membrane. This suggestion offered as a possible mechanism of action is supported in part by the fact that microscopic examination of the stained ascitic fluid taken from the mice after four treatments with the mixture of vitamins C and B12 revealed many lymphocytes, neutrophils, monocytes, and disintegrating tumor cells. When the

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Days after Ehrlich transplant

Fig. 2. Survival o f mice bearing Ehrlich carcinoma after treatment with vitamins C and B 1 2 . 100 mice were sampled; 50 treated (•) and 50 control (o). + = Terminated.

Influence o f Vitamins C and B |2 on Survival

Acknowledgements This work was supported in part by a grant from the Pennsylvania Division o f the American Cancer Society, Hershey, Pa. We wish to thank the following for generously providing tumor stock mice: Dr. D. Houchens, Battelle Laboratories. Columbus, Ohio: P388 leukemia; Dr. T. Emmet, Kettering Laborato­ ries, University o f Cincinnati, Ohio: Ehrlich carcino­ ma. We acknowledge with gratitude the donation of 20 g B 12 (hydroxocobalamin) from Merck Chemical Company.

References 1 Basu. T.D.; Dickerson, J.W.T.; Williams, D.C.: Interrelationships o f nutrition and cancer. Ecol. Food Nutr. 2: 193 (1973). 2 Basu, T.D.: Significance o f vitamins in cancer. Oncology 33: 183-187 (1976). 3 Benadc, L.; Howard, T.; Burk. D.: Synergistic kill­ ing of Ehrlich ascites carcinoma cells by ascorbate and 3-amino-1,2,3,-triazole. Oncology 23: 33-43 (1967). 4 Cameron, E.; Campell, A.: The orthomolecular treatment o f cancer. II. Clinical trial o f high dose ascorbic acid supplement in advanced human can­ cer. Biol. Interact. 9: 285-315 (1974). 5 Cameron. E.; Pauling, L.: Supplemental ascorbic acid in the supportive treatment o f cancer: prolon­ gation o f survival times in terminal human cancer. Proc. natn. Acad. Sci. USA 73: 3685-3689 (1976). 6 Cameron, E.; Pauling, L.: The orthomolecular treatment o f cancer. I. The role o f ascorbic acid in host resistance. Biol. Interact. 9: 273-283 (1974).

7 Cameron, E.; Pauling, L.; Leibovitz, B.: Ascorbic acid and cancer: a review. Cancer Res. 39:663-681 (1979). 8 Cohen, M.H.; Carbone, P.P.: Enhancement of the anti-tumor effects o f l,3-bis-(2-chloroethyl)-l-ni­ trosourea and cyclophosphamide by vitamin A. J. natn. Cancer Inst. 48: 921 (1972). 9 Copeland, E.M.; Daly. J.M.„ Dudrick, S.J.: Nutri­ tion as an adjunct o f cancer treatment in the adult. Cancer Res. 37: 2451-2456 (1977). 10 Danilevicius, Z.: Cancer and nutrition. J. Am. med. Ass. 238: 1182-1183 (1977). 11 Dresser, D.W.: Adjuvanticity o f vitamin A. Na­ ture, Lond. 217: 527 (1968). 12 Kaplan. S.S.; Busford, R.E.: Effect o f vitamin B |2 and folic acid deficiencies on neutrophil functions. Blood 47: 801-805 (1976). 13 Lewin, S.: Evaluation o f potential effects of high intake o f ascorbic acid. Compar. Biochem. Physi­ ol. 47: 681-695 (1973). 14 Old, L.J.: Cancer immunology. Scient. Am. 236/5: 62-69(1977). 15 Park, C.H.; Amare, M.; Savin, M.A.; Hoogstraten, B.: Growth suppression o f human leukemia cells in vitro by ¿-ascorbic acid. Cancer Res. 40: 1062: 1065 (1980). 16 Poydock, M.E.: Fardon, J.C.; Gallina. D.; Ferro. V.: Heher, C.: Inhibiting effect of vitamins C and B 12 on the mitotic activity o f ascites tumor. Expl Cell Biol. 47: 210-217 (1979). 17 Poydock. M.E.; Reikert, D.; Rice, J.; Aleandri. L.: Inhibiting effect o f dehydroascorbic acid on cell division in ascites tumors in mice. Expl Cell Biol. 50:34-38 (1982).

Received: June 26, 1981 Accepted: July 24. 1981 Sister M. Eymard Poydock, PhD, Mercyhurst College. 501 East 38th Street, Erie, PA 16546 (USA)

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stained ascites fluid was examined after the sixth and seventh treatments, no tumor cells, but an abundance of monocytes and macro­ phages were found [16]. This possible expla­ nation, however, is hypothetical and cer­ tainly needs to be verified.

91

Expl Cell Biol. 47: 210-217 (1979)

Inhibiting Effect of Vitamins C and B12 on the Mitotic Activity of Ascites Tumors M. Eyrnard Poydock, J.C. Fardon, D. Gallina, V. Ferro and C. Heher Mercyhurst College, Erie, Pa. and St. Thomas Institute, Cincinnati, Ohio

K ey Words.

Vitamin B l2 • Vitamin C • Calcium ascorbate • Mitoses • Cancer

Abstract. The mitotic activity of the transplantable mouse tumors, Sarcoma 37, Krebs-2, and Ehrlich carcinomas, in the ascites form, were inhibited after treatment with a mixture of vitamins C and B15 with no apparent toxic side effects. These vitamins when administered alone, at the same dosage, did not seem to have any apparent effect on mitosis or the morphology of the cells studied. Microscopic examinations of the stained ascites fluid taken from the mice treated with the vitamin mixture showed few tumor cells, and these in various stages of disintegration. Also, an increase in lymphocytes, monocytes and neutro­ phils were noticed; however, later in the experiment, no tumor cells could be found and monocytes and macrophages were abundant.

Indroduction

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Since chemotherapeutic agents such as antimetabolites and alkylating agents have proven to be cytotoxic for both malignant and normal proliferating cells, the authors thought it advisable to investigate some of the natural com­ pounds which might exhibit selective action without the associated toxic side effects. Current animal experimentation and clinical findings suggest a new dimension in the treatment of cancer. Besides surgery, radiotherapy, chemother­ apy, and immunotherapy, nutritional therapy is finally coming into its own (12). There is creeping into the literature a steady increase in the number of studies on nutrition, diet, vitamins, and their relation to cancer. Preliminary in vitro studies conducted on the mitotic activity of Sarcoma 37, L1210 leukemia, and Ehrlich

Inhibiting Effect of a Vitamin C and Bls Mixture

211

carcinoma, after treatment with a mixture of vitamin C and vitamin Bl2, demon­ strated almost complete inhibition of mitosis. No regression of mitosis, however, was noted in the nonneoplastic L929 fibroblasts that were tested at the same time. Early experiments indicated that undernourished rats did not accept tumor transplants as readily as the well-nourished animals. The tumors that did develop were smaller than in the well-fed controls (5,20,22). More recently the effect of nutritional therapy in children and adults with cancer has been evaluated (11, 24). The importance of diet and its relation to cancer has been reported by several researchers (3, 21, 23). A number of workers (2, 10, 13) have explored the efficacy of vitamin A treatment alone, and in combination with anticancer agents. Clinical findings by Cameron and Pauling (8), as well as other researchers, support the general contention that large doses of vitamin C enhance natural resistance to cancer, and prolong the survival times in terminal human cancer patients (8, 9, 15). Burns et al. (7) noted that the carcinogen methylcholanthrene evoked an increase of ascorbic acid (vitamin C) in experimental animals. More recently, several investigators report that the majority of patients with malignant diseases have minimal tissue storage of vitamin C (1, 14). Benade et aL (4) reported that the cytocidal property of sodium ascorbate is believed to be due in part to the intracellular generation of hydrogen peroxide upon oxidation. With the exception of vitamin C, a search of the literature revealed that the relationship between vitamins and cancer is a relatively unexplored field. Some unpublished preliminary experiments performed in this laboratory also indicated a possible effect of vitamin B12 when mixed with vitamin C. For these reasons it seemed of interest to extend the in vitro studies to include an examination of the effect of the vitamin mixture on the mitotic activity of various ascites tumors in vivo.

Materials and Methods

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Preparation o f Vitamin Mixture The vitamin C (ascorbic acid) and its salt calcium ascorbate (CaAs) were purchased from Sigma Chemical Co. Vitamin B13 (hydroxycobalamin) was obtained from Merck Chemical Division. Cyanocobalamin was found to be equally effective. A solution was prepared by adding 0.1 g B„ to 10 ml distilled water. To this solution 0.2 g ¿-ascorbic acid was added. Immediately before use the mixture was diluted as follows: one part vitamin C and Bl2 mixture was added to 3 parts of 5% calcium ascorbate.

Poydock/Fardon/Gallina/Fcrro/Heher

212

In vitro Experiments Sarcoma 37, Ehrlich carcinoma, Krebs 2 and L1210 leukemia were chosen as neoplas­ tic material, obtained from various sources (see Acknowledgements). Fibroblasts L929 (Microbiological Associates, suspensions in frozen ampule) were selected as the nonneoplastic cells. The cultures were propagated in 30-ml Falcon flasks containing 4 ml MEM Eagle, Earle’s base medium with 10% calf serum. For testing the effect of vitamin C and Bn mixture on the neoplastic cells, the ascites exudate was removed from the tumor-bearing mice. It was washed with saline, centrifuged at 500 rpm for 10 min and the pellet resus­ pended to bring the cell suspension to 106 cclls/ml. Immediately 0.025 ml of the vitamin mixture was introduced into the test flasks. Both these and the control flasks were incubated at 37.5 °C. Mitotic counts were made by withdrawing a drop of the cell suspension with a Pasteur pipette and staining with acetocarmine. About 500 cells were counted. Paul’s method (17) for determining the mitotic índex (MC) was used: M

C

Number of mitotic cells :---------- :----------7 — r.------------ — r X Total number of cells counted

= = —

_____ 1 ,0 0 0 .

For estimating the effect of the vitamins on the mitotic activity ofL929 fibroblasts, the proce­ dure was the same, except that counts were made every hour in approximately 10 fields at 300X. Using an inverted microscope with phase optics, only metaphases, anaphases, and telophases were counted because of the uncertainty of detecting cells in prophase under phase.

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In vivo Experiments The animals employed in this investigation were female HA/ICR Swiss obtained from Roswell Park Memorial Institute. The transplantable Sarcoma 37, Krebs 2, and Ehrlich carcinomas, in the ascites form, were used. To carry the ascites tumors in stock mice they were injected (i.p.) with 0.1 ml (10®) washed ascites cells (19) usually harvested on the 7th day after tumor implantation. For testing the above tumors a total of 50 mice was used for each type. Five separate experiments were performed for each type of tumor. 24 h after tumor implantation the mice were divided equally into two groups and designated as test and control. The test animals were treated (i.p.) daily with 360 mg/kg body weight vitamin C and B, 3 mixture for 7 successive days. As the mice were uniformly of 25 g weight, this meant that each time 0.2 ml of the solution was injected into each of them. The control animals received an equal volume of Hank’s saline. Starting after the third treatment, and continuing for 5 successive days, one test and one control mouse was sacrificed, by cervical dislocation, at the same time each day. It has been known for some time that there is a daily periodicity of mitosis in tissues of various laboratory animals (6, 16). The ascites exudate was removed and the body cavity washed three times with physiological saline. The ascites fluid plus the washings were placed in a graduated tube, centrifuged at 500 rpm for 10 min, and the packed cell volume (PCV) recorded. The resuspended pellet was used to prepare an acetocarmine smear using the procedure described previously (18). For determining the mitotic index approximately 500 tumor cells were counted at a magnification of 675X. For ease in counting the cells a Howard disc was placed in the ocular of the microscope. The mitotic index (M/C) was determined as indicated above. A subsequent scrutiny was con­ ducted to determine the effect of various dosages of vitamin C, administered alone, on mitotic activity of ascites tumor. Also a similar study, with B,¡ alone, was performed.

213

Inhibiting Effect of a Vitamin C and B,2 Mixture Results

The in vitro results, typical of many experiments, are shown in table 1. The tests demonstrated that the vitamin C and B12 mixture inhibited mitoses in all neoplastic tissues but division of the nonneoplastic L929 fibroblasts were not affected. Table II presents the in vivo results after treatment with the vitamin mix­ ture. The table represents the average of 5 experiments done with each type of tumor. The PCV of the ascites exudate taken from the treated mice was insignificant; in fact, only a tliin film of cells was noted in the bottom of the centrifuge tube. Using a light microscope, an examination of the acetocarmine smear prepared from the film showed few tumor cells and no mitotic figures.

I. Effect of a vitamin C and Bu mixture on mitoses of malignant and nonmalignant cells in vitro Table

Incubation time h

Mitotic index test

control

Sarcoma 37

0 5 6 8.5

48 0 0 0

48 30 75 86

Ehrlich

0 3.5 4.5 6.5

78 0 0 0

78 41 55 63

L1210

0 2.5 4.5 6.5

46 0 0 0

46 40 42 48

L929

0 2.5 4.5 6.5

52 50 62 64

52 48 60 62 Downloaded by: Stockholm University Library 130.237.165.40 - 5/6/2019 4:48:55 AM

Tissue tested

214

Poydock/Fardon/Gallina/Ferro/Heher

Mitotic counts (M/C) and packed cell volume (PCV, in ml) taken for 5 successive days after treatment with vitamins C and B Table II.

Number of treatments

Krebs

Sarcoma 37

Ehrlich M/C

PCV

M/C

PCV

M/C

PCV

T C

T C

T C

T C

T C

T C

3 4 5 6 7

0 0 0 0 0

40.5 39.6 45 31.6 28

0 0.5 0 0.96 0 1.24 0 1.54 0 2.1

0 0 0 0 0

0 0 0 0 0

0 0 0 0 0

0 0 0 0 0

Mean

0 36.9

0 1.27

0 26.7

25.2 32 25.6 25.6 25.2

0.96 0.92 0.7 0.94 0.9

0 0.89

29.6 22 21 20 24

0 23.3

1.34 0.66 0.72 0.72 1.4

0 0.97

The table represents the average of five experiments performed with each type of tumor. T = test; C = control.

Mitotic counts taken for 5 successive days in Ehrlich ascites tumor after treatment with vitamin C and B,2 administered separately Table III.

Vitamin C and CaAs 340 mg/kg

10% vitamin C 800 mg/kg

Vitamin B,, 20 mg/kg

test

control

test

control

test

control

3 4 5 6 7

29.2 48.8 45.2 36.8 39.6

53.2 45 50 41 53.2

11.4 12.4 17.6 32.8 19

38.8 42 47.6 38 37.6

39.2 36.4 36 35.6 47.6

34 32.4 49.6 34 35.6

Mean

39.9

48.9

18.6

40.8

38.9

37.1

Number of treatments

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The table represents average data of five experiments performed with each type of treat­ ment.

Inhibiting Effect of a Vitamin C and B,3 Mixture

215

The tumor cells appeared to be in various stages of disintegration; however, many lymphocytes, neutrophils and monocytes were present. Toward the end of the experiment no tumor cells, but an abundance of monocytes and macro­ phages were found in the ascitic fluid. Table III shows the mitotic index of tumor-bearing mice treated with vitamin B12 and various dosages of vitamin C. Both vitamins, although adminis­ tered separately, were given at the same dosage as in the mixed preparation (i.e. vitamin C and calcium ascorbate 340 mg/kg, vitamin B12 20 mg/kg). Neither B i2 nor vitamin C had any apparent effect on mitoses. It should be noted, however, that when a higher dosage of vitamin C was administered (800 mg/kg) mitosis was slightly inhibited. At a dosage of 1,600 mg/kg vitamin C proved to be toxic to mice.

Discussion and Conclusion

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The present research demonstrated that after three injections of a mixture of vitamin C and B12 the mitotic activity of the ascites tumor cells (Sarcoma 37, Krebs 2, and Ehrlich carcinoma) was stopped. A microscopic scrutiny of the acetocarmine smear, prepared from ascitic fluid taken from the treated mice, revealed a tremendous increase in lymphocytes, neutrophils and monocytes. This was followed by disintegration of tumor cells and an increase in monocytes and macrophages. More often there were no tumor cells found even after the third treatment. Observations such as these, suggest that the vitamin mixture may enhance the host’s immune mechanism. Yamufuji et al. (25) reported the antitumor potency of ascorbic acid, while other workers supported the view that vitamin C stimulates the natural resistance of the host (2 ,8 ,9 ). Results obtained with small daily doses of vitamin C (340 mg/kg) demonstrated no apparent effect on the mitotic activity of tumor cells in vivo, nor any increase in the white blood cell population of the ascitic fluid, although the mice received the identical amount of vitamin C present in the vitamin mixture. When higher doses of vitamin C (800 mg/kg) were tested, mitoses were reduced. Experiments done in these laboratories disclosed that Celite (diatomaceous earth) injected intraperitoneally, also produce an influx of both mature and immature white blood cells into the peritoneal cavity. Subsequent results disclosed no inhibition of tumor cells after several injections of Celite. It appears to be evident from the above in vivo and in vitro experiments that the vitamin mixture has a specific effect in that it inhibits mitotic activity of

Poydock/Fardon/Gallina/Ferro/Heher

216

neoplastic cells in vivo and in vitro wliile it did not inhibit mitoses of normal cells. An examination of the stained ascitic fluid taken from the treated mice often revealed white blood cells in division, indicating that the normal flora of the peritoneal cavity was not affected by the treatment. Further investigations are required to determine the precise nature of this activity. For example, a synergistic action or some type of interaction between the two vitamins may be involved.

Acknowledgements We are grateful to the following for generously providing tumor material: Dr. A. T. Bumess, Sloan-Kettering Institute for Cancer Research, Rye, N.Y. (Krebs 2 in stock mouse); Dr. D. Houchens, Battelle Laboratories, Columbus, Ohio (L I210 stock mouse); Dr. T. Emmet, Kettering Laboratories, University of Cincinnati, Cincinnati, Ohio (Ehrlich carcino­ ma in stock mouse). Sarcoma 37 was purchased from Jackson Memorial Laboratory, Bar Harbor, Me. in a stock mouse.

References

3 4 5 6 7

8

9

Basu, T.D.: Significance of vitamins in cancer. Oncology 33: 183-187 (1976). Basu, T.D.; Bishun, N.P., and Williams, D.C.: Accentuation of the cell killing effects of chlorambucil by phénobarbital, caffeine and vitamin A in culture. Cytobios 9: 115 (1974). Basu, T.D.; Dickerson, J.W.T., and Williams, D.C.: Interrelationships of nutrition and cancer. Ecol. Food Nutr. 2: 193 (1973). Benade, L.; Howard, T., and Burk, D.: Synergistic killing of Ehrlich carcinoma cells by ascorbate and 3-amino-l,2,4 triazole. Oncology 23: 33-34 (1969). Bishoff, J. and Long, M.L.: The influence of calories per se upon the growth of Sarcoma 180. Am. J. Cancer 32: 418 (1938). Bullough, W.S.: Energy relations of mitotic activity. Biol. Rev. 27: 133-168 (1952). Burns, J.J.; Conney, G.D.; Peter, G.D.; Evans, C.; Martin, G.R., and Taller, D.: Observations on the drug induced synthesis of D-glucuronic, /.-gulonic and /.-ascorbic acids in rats. J. Pharmac. exp. Ther. 129: 132-138 (1960). Cameron, E. and Pauling, L.: Supplemental ascorbate in the supportive treatment of cancer: prolongation of survival times in terminal human cancer. Proc. natn. Acad. Sci. USA 73: 3685 - 3689 (1976). Cameron, E. and Campbell, A.: The orthomolecular treatment of cancer. II. Clinical trial of high-dose ascorbic acid supplements in advanced human cancer. Biol. Interact. 9: 285-315 (1974). Downloaded by: Stockholm University Library 130.237.165.40 - 5/6/2019 4:48:55 AM

1 2

Inhibiting Effect of a Vitamin C and B12 Mixture

11 12

13 14 IS

16 17 18 19 20

21 22

23 24 25

Cohen, M.H. and Carbone, P.P.: Enhancement of the anti-tumor effects of 1,3-bis(2-chloroethyl)-l-nitrosura and cyclophosamide by vitamin A. J. natn Cancer Inst. 48: 921 (1972). Copeland, E.M., III; Daly, J.M., and Dudrick, S.J.: Nutrition as an adjunct to cancer treatment in the adult. Cancer Res. 37: 2451-2456 (1977). Copeland, E.M., III; McFadyen, B.V., jr., and Dudrick, S.J.: Intravenous hyperalimen­ tation in cancer patients. J. surg. Res. 16: 241-247 (1974). Dresser, D.W.: Adjuvanticity of vitamin A. Nature, Lond. 217: 527 (1968). Krasner, N. and Dymock, I.W.: Ascorbic acid deficiency in malignant diseases: a clinical and biochemical study. Br. J. Cancer 30: 142-145 (1974). Lewin, S.: Evaluation of potential effects of high intake of ascorbic acid. Comp. Biochem. Physiol. 47: 681-695 (1973). Movchan, O.T.: The daily periodicity of the mitotic activity in corneal epithelium of starving rats and mice. Biull. Eksptl. Biol. Med. 52: 845-848 (1961). Paul, J.: Cell and tissue culture, pp. 256-257 (Williams& Wilkins, Baltimore 1961). Poydock, M. Eymard, and Fardon, J.C.: The effect of the presence of Sarcoma 37 on corneal epithelium of mice. Growth 28: 127-133 (1964). Roitt, I.M.: Washing of cells. Biochem. J. 63: 300 (1956). Rous, P.: The influence of diet on transplanted and spontaneous tumors. J. exp. Med. 20: 433 (1914). Sporn, M.B.: Nutr. Rev. 35: 65-69 (1977). Sugiura, K. and Benedict, S.R.: Athreptic theory of growth of cancer. J. Cancer Res. 10: 194 (1926). Tannenbaum, A. and Silverstone, H.: Nutrition and genesis of tumours. Cancer 1: 306 (1957). Eys, J. van: Nutritional therapy in children with cancer. Cancer Res. 37: 2457-2461 (1977). Yamafuji, K..; Nakamura, Y.; Omura, H.; Soeda, T„ and Gyotoku, K.: Anti-tumor potency of ascorbic, dehydroascorbic, or 2, 3-diketogulonic acids and their action of deoxyribonucleic acid. Z. Krebsforsch. 76: 1-7 (1971).

Received: May 9, 1978; accepted: June 30, 1978 Sister M. Eymard Poydock, PhD, Mercyhurst College, 501 East 38th Street, Erie, PA 16501 (USA) Downloaded by: Stockholm University Library 130.237.165.40 - 5/6/2019 4:48:55 AM

10

217

Effect of combined ascorbic acid and B-i 2 on survival of mice with implanted Ehrlich carcinoma and Li 2i 0 leukemia1’2 M Eymard

Poydock

ABSTRACI’

A combination

hydroxycobalamin in

mice.

these

The vitamins

leukemias. After

kg body

assay

wt) of the

vitamins

cells, but not in normal ascorbate plus vitamin

that

with

vitamin

daily

for

with

and

of tumors

cobalt

C, forming plus

ip tumor 0.2

mL

cobalt

g/ by

alive after 60 d. In in Ll2 10 leukemia research with cobaltthat when B-l2 is

nucleus

of B-l2

ascorbate.

C also

vitamin

cells died

attaches

Tests

inhibited

carcinoma,

Krebs

various

(0.4

10 d. All controls

mice were of mitoses

C, the

ascorbate

Am

0. 1 mL

injected

L929 cells. In recent C, we demonstrated

on vitamin

cobalt

cells.

acid

mitoses

received

were

50% of the treated revealed inhibition

>

to a carbon

inhibited

40 mice

24 h, 20 mice

day 19, but vitro findings

combined

B-l2)

present study was performed to test the effect of on the survival of mice bearing carcinomas and

In each

(X105).

of dehydroascorbic

(vitamin

WORDS vitamin

Ascorbic

acid,

B- 12, cancer,

ing 0. 1 g B-l2

three parts The tumor tumor

was

times and

M/C

therapy

ating

agents

can

be cytotoxic

well as malignant requires

as antimetabolites

for

normal

cells. Consequently,

an exploration

of other

response malignant,

modifiers and agents but not to normal

has been

conducted

in our

approaches,

and

totic

dehydroascorbic

activity

of several

fibroblasts. firmed

This

the selective

followed

effect

by investigations

tumor after explorations mor

types

inhibition

of tumor

by these

Inhibition acid

and

1 shows

obtained

cells,

but

by experiments

on the

not

on normal

in mice

These

preliminary

ofthe

survival

ofmice

mi-

that con-

results

bearing

the vitamin mixture. Finally, to elucidate the mechanism

day

50 mice tumor

ascites implantation.

of the

ascites

animals

further of tu-

third

groups. vitamin received

an and

HA/ICR Institute

2, and

Mice

were

10 mm

smear

and

were

as described

on the using

experiments divided

equal

volume remaining

into wt,

of Hank’s

equal with for

resuspended

of dehydroascorbic

acid

to prepare

Similar alone

studies

and

ip

After

the

and

one

The ascites at 500 rpm acetocarmine

examined

vitamin

and

mL

7 d. Control

control

an

of

after

test 0.2

saline.

5 d, one

seventh

hours

daily

body

carwith

a total

Twenty-four

treated

mg/kg

for the

above.

Ehrlich injected

Five were

Swiss (Buf-

guidelines of the use of laboratory

(X 106), harvested

were

360

Krebs

type.

The inhibited

the nonneoplastic

test animal were killed at the same time each day. exudate and body cavity washings were centrifuged

the

effect

B- 12 alone.

in vitro and in vivo by dehydroascorbic From

In vitro experiments.

Address

Am J C/in Nutr

mice

X 1000

B-12

to the and

used.

tumor

animals

mixture,

treatment,

I

For

cells

the Test

37,

were

for each

implantation

control

were

agents.

form

were

as follows:

experiments.

vitamin

care

a drop cells

counted)

but did not affect

sarcoma

used

500

calculated

in vitro

conformed for the

a the

to 106 cells/

1) by staining

cells

and

and

was added to the counts were made

The animals were female Roswell Park Memorial

the

tumor were

was

of the

cells

10 mm,

About

cells/total

acid

ip washed

after

2

(1).

from

in the ascites

B-12

for

(Table

(M/C)

results

falo, NY). All procedures National Research Council

B-12

elsewhere

the

Transplantable

in vitro

index

of dehydroascorbic

cinomas

vitamin

rpm

acetocarmine.

of mitotic

animals.

of experiments

and results ofmiiosis

mitotic

In vivo experiments. mice

for

Methods

the

= (number

0. 1 mL and

part vitamin mixture. exudate taken from

the cell suspension

the day

with

a series

in vivo.

treatment with were conducted

biological

throughout

mitoses in the neoplastic L929 fibroblasts.

as

therapy

effect

to 10 mL

at 500

to bring

be selectively toxic to latter line of research

an inhibiting

acid

was followed

alkylcells

in cancer including

that might cells. The

are summarized below. Early experiments demonstrated ofcombined

proliferating

progress

laboratory,

and

effective)

at various

Introduction such

was equally

tumor

Table

agents

medium by add-

centrifuged

cell suspension

from nonneo-

Earle’s base was prepared

ascorbate to one from the ascites

resuspended

obtained for the

in Eagle, mixture

proved

mixture

Chemotherapeutic

mouse,

were

obtained

0.2 g dehydroascorbic acid (see use the mixture was diluted with

adding before

5% calcium was obtained

stock

pellet

C, hydroxycobala-

vitamin

were

(cyanocobalamin

distilled water, and then Appendix). Immediately

of the

combination

L929

plastic cells. Cells were propagated with 10% calfserum. The vitamin

counted mm,

L 1 2 1 0 leukemia

fibroblasts

mL. Then, 0.025 mL of the vitamin mixture test flasks. After incubation at 37.5 #{176}C, mitotic

J C/in Nuir 199 l;54:l26lS-55.

KEY

2, and

sources;

These the

l99I;54:

neoplastic 1261S-SS.

results

have

material, Printed

been

reported

sarcoma in USA.

in detail 37,

Ehrlich

© 1991 American

Downloaded from https://academic.oup.com/ajcn/article-abstract/54/6/1261S/4715221 by guest on 02 April 2018

Unit,

Society

the Cancer reprint

Mercyhurst

for Clinical

Research requests

College,

Nutrition

Institute,

to ME Poydock, 501

East

38th

Mercyhurst

College,

Erie, PA.

Director

ofCancer

Research

Street,

Erie, PA 16546. 12615

12625

POYDOCK

TABLE 1 Effect ofa mixture

ofdehydroascorbate

and vitamin

(hydroxycobalamin)

on mitoses

of malignant

Ehrlich,

nonmalignant

cells

L I 2 10) and

B-l2

cells

(L929

37,

(Sarcoma

fibroblasts)

Tissue

tested

2

Mitotic

counts

S successive

in vitro*

Mitotic

Incubation time

TABLE

acid

and

Ehrlich

index

Test

37

Ehrlich

L12l0

L929

*

Reference

M/C

PCV

T

C

T

C

T

C

I

many

tumor cells. were visible

0

40.5

0

0.5

0

25.2

0

0.96

0

29.6

0

0

39.6

0

0.96

0

32.0

0

0.92

0

22.0

0

1.34 0.66

0 0

75 86

5

0

45.0

0

25.6

0

0.7

0

21.0

0

0.72

0

25.6

0

0.94

0

20.0

0

0.72

78

78

7

31.6 28.0

1.54

0

0 0

0 0 0

1.24

6

2.1

0

25.2

0

0.9

0

24.0

0

1.4

3.5 4.5 6.5 0 2.5 4.5 6.5 0 2.5 4.5 6.5

0 0 0 46

41 55 63 46

Mean

0

36.9

0

1.27

0

26.7

0

0.89

0

23.3

0

0.97

0

40

0 0 52 50 62 64

42 48 52 48 60 62

ofthe with

the results

stained

ascitic

the vitamin monocytes,

fluid

were

observed.

ofthe

effects

from

the

mixture revealed and disintegrating

the average

Represents

*

T, test: C, control.

were

used.

the

Test

mixture.

over,

of five experiments

Reference

performed

on mitoses;

the data

of the tumor from mice

treated with the vitamin were few tumor cells and

mixture no mitotic

trophils, and with reduced

monocytes ascorbate,

were abundant. In contrast, treatment calcium ascorbate, and vitamin B- 12

administered mixture, had

separately, no effect

tumor

3). Injecting

(Table

slightly These

inhibited results

mixture tivity

the

carcinoma) ofthe

ascites tumors was completely

mice

cytes,

L929

neutrophils,

suggesting ascorbic activity

or

droascorbic cites tumors due

white

monocytes,

were

treated

daily

Animals

were

killed

after

that

it was

not

three

by the ascites

injections B-l2,

and

Ascites

inhibition By using

fluid

whereas packed-cell Few tumor cells and

TABLE

3

Mitotic

counts

after

tumor

we

investigated

the

acid on mitotic activity oftwo (2) and attempted to determine acidity

of dehydroascorbic

of animals exceptions.

were the same P388 leukemia

effect

different whether acid.

as described and Ehrlich

of dehymurine asthe effect

The

methods

above, with carcinoma

Downloaded from https://academic.oup.com/ajcn/article-abstract/54/6/1261S/4715221 by guest on 02 April 2018

seven

*

treatments.

acidity

treated acid.

More-

of dehydroas-

with citric Results

alone

4). The

ascites

acid at proved

dramatically

exudate

macrophages

were

animals was was observed,

3-5 mL. whereas

numerous.

tumor

acid

Ascorbic acid (800 mg/kg)

Vitamin B-l 2 (20 mg/kg)

C

T

C

T

C

3

29.2

53.2

1 1.4

38.8

39.2

34.0

4

48.8

45.0

12.4

42.0

36.4

32.4

45.2 36.8 39.6 39.9

50.0 41.0 53.2 48.9

17.6 32.8 19.0 18.6

47.6 38.0 37.6 40.8

36.0 35.6 47.6 38.9

49.6 34.0 35.6 37.1

Represents

average

data

of five experiments

performed

with

of treatment. as in the vitamin ascorbic in the

each

The vitamin B-12 was administered in the same dosage mixture whose results were presented in Table 1; the acid/calcium ascorbate was also in the same dose as that used

type

acid

af-

from

and only a thin film ofcells could of the tube after centrifugation,

volume of control no mitotic activity

T

S 6 7 Mean

cells,

mL

of

treatments

function. Injections of had no effect on mitotic

10 mg/0.2

separately*

ac-

from

of tumor.

of mitoses, a subsequent cxthe same procedure as above,

(Table

and calcium asconbate (340 mg/kg) No.

type

taken for 5 successive days in Ehrlich ascites with ascorbic acid and hydroxycobalamin

treatment

administered

of lympho-

disintegrating

and

monocytes

Ascorbic

Ehrlich activity

taken

activity

was insignificant, in the bottom

of the

mitotic

concentration

of immune B- 12 alone

mice

ascorbate

37, Krebs 2, and whereas the mitotic not.

mitotic

be observed

with

the

citric acid had no effect on mitoses. Treatment with dehydroascorbic acid the

each

acid ip for 7 d, and control animals received of saline. Vitamin B- 1 2 was not included in

to demonstrate

treated

blood cell population.

experiments,

to the

and number the following

was

of reduced

vitamin

an increased

an enhancement acid or of vitamin

In further

after and

(sarcoma inhibited,

fibroblasts

contained

dose

that acid

there neu-

doses as provided counts of Ehrlich

a higher

mitoses. demonstrated

normal

treated

was

in the same the mitotic

of dehydroascorbic of

was insignificant, and figures. Lymphocytes,

that

with

1.

animals

dehydroascorbic an equal volume

fected

no tumor cells but numerous

represent an average of five experiments for each types. Packed-cell volume of the ascites exudate

on

C

3

macrophages

2 presents

I

4

neutrophils,

and

Table

C

30

By the sixth and seventh treatments, in the ascitic fluid of treated mice,

monocytes

T

48

treatments

lymphocytes,

C

0

corbic acid that caused periment was performed.

four

PCV

48

examination

after

M/C

0 S 6 8.5

1.

Microscopic

Krebs

PCV

test animals injected with tumor were the same pH (2.4) as the dehydroascorbic mice

37

for

of

treatments

/1

Sarcoma

M/C

Control No.

Sarcoma

(M/C) and packed cell volume (PCV, in mL) taken days after treatment with a mixture of dehydroascorbic hydroxycobalamin (vitamin B12)*

vitamin was

tested.

mixture,

but

ascorbic

T, test; C, control.

acid

Reference

rather

than

1.

dehydnoascorbic

COMBINED TABLE 4 Mean mitotic after treatment

carcinoma and P388 leukemia acid (DHA) and citric acid* DHA, 400 mg/kg (pH 2.4) Ehnlich carcinoma

mg/kg (pH 2.4) P388 leukemia

Mean Each

C AND

B-12

Effect index taken in Ehnlich with dehydroascorbic

DHA, 400

*

VITAMINS

showing

increased

hydroascorbic

acid

corbic

acid

kemia

(4).

and

hearing Earlier any

29

ducted.

37

34

34

40 female DBA/2 mice used in each: the third experiment involved ascorbic acid, with 60 mice used. Mice were injected

39

0

0

38

0

T

of 50 mice

(25 test,

25 control).

from

Roswell

A total

Two

tion;

cells.

effect

this

and form

cells

fibroblasts

or white

results

have

been

and

before

0.01

P388

g sodium

injection,

reported

used.

as follows:

and was

effects tumors.

elsewhere

(3).

Ehrlich

without blood

would

with

maintained

be

These

carcinoma

in the

in DBA/2

was maintained vitamin mixture

stock

in HA/ICR Swiss was prepared by

0.0 1 g B- 12, 0.0 1 g dehydroascorbic

ascorbate,

1 mL

these

ofanimals

leukemia

were

the crystals

whether

P388

and Ehrlich carcinoma In this experiment the

adding acid,

of malignant

survival

of

(1 , 2) demonstrated

division

sterile

into

a small

tube.

Immediately

distilled

water

was

added

to the

tube.

Two

experiments

in BDF1 mice;

mice 100

were

and

mice

one (50

conducted,

with test,

Ehrlich

mice

received

for 8 consecutive of saline.

On

days. the

mL

Control

second

were

ip with divided

6 mg/0.2

with

P388

carcinoma

50 control)

ment. Animals were injected (X 106). After 24 h, mice were Test

one

leukemia

in HA/ICR used

animals

weekend,

received

mice

with

acid

oftumor-bearing

with tually

P388

and

Figure

mice.

leukemia,

identical.

but

P388

and

were

terminated

Ehrlich after

and

vitamin

B-l2

mice

carcinoma, -.-70

the results

B-l2

d, at which

for mice

bearing

P388

time

were still these results

>

with

among control were still alive Postmortem vealed mice

in 0. 1 mL which mice

mice

were

were

injected

with

Tyrodes later

solu-

progressed

injected

with

or later they for a total

under

dehydroascorbic increase

the

vitamin

The

same

imals

and

histological disease

was

that

our

B-l2

median

animals was 12 d, whereas for treated when the experiment was terminated

disseminated killed

plus

in survival.

after

day

examination to liver,

60 revealed

and

no evidence

minimal, earlier

and

all animals

published

papers

were

mice after

>

50% 60 d.

mice

lungs:

re-

treated

of disease.

dead

referred

pro-

survival

of control

spleen,

In the experiment involving recently purchased plus B- 1 2, the increase in survival of treated over

ascorbic untreated

by day

acid an-

1 7. (Note

to ascorbic

acid,

but

100

90#{149} 80

treated were virwere

animals

alive. demonstrate

a very

in survival of tumor-bearing with a mixture ofdehydroascorbic

animals acid

(hydroxycobalamin).

Downloaded from https://academic.oup.com/ajcn/article-abstract/54/6/1261S/4715221 by guest on 02 April 2018

.-

-1

70 60 50 40 as

30

;

20

C

>1 > cn

0

0

experiments

50% ofthe

Control

a substantial

volume

leukemia

I S d; the

10 cells leukemia, 24 h, test

conacid,

acid

duced

daily

deaths in the vitamin-treated survival for untreated mice, was

After

experiments

Treatment

the survival

carcinoma

Ll2

three

dehydroascorbic

volume, pH, and osmolarity. dehydroascorbic acid, each results, shown in Figure 2.

no treatment,

increased

for Ehrlich

the earliest 32. Median

receiving the vitamin treatment Whatever the mechanism, significant improvement treated intraperitoneally

1 shows

results

All 50 control

dead by day 17, whereas group occurred on day both

B- 12 dramatically

l0

an ascitic

disease.

10 treatments.

and then injections were continued on alternate days for 3 wk for a total of 1 7 treatments. Treatment with a mixture of dehydroascorbic

involved

experi-

an equal

received

Institute. in the

Swiss

in each

C and

used

conditions with saline ofthe same The two experiments involving with 40 mice, produced identical

0. 1 mL washed ascites cells into test and control groups. ip vitamin

ascorbate, dissolved with was supplied in DBA/2

Memorial were

experiments

produced

was prepared in crystalline 0.01 g dehydroascorbic acid,

10 mg/0.2 mL ip (0.4 g/kg body wt) dehydroascorbate/B-l2 ascorbic acid/B-12 for 8 consecutive days. Two days were given another two treatments on alternate days,

of mice

of normal

to be answered

Transplantable mice, mice.

cell

in increased

methods ascites

on

inhibition

It remained

manifested

on survival

Park

of 140 mice

intraperitoneally

2.

tumor

comparable

leu-

32

0

an inhibitory

L12 10 mouse

36

34

in vivo

de-

mice

50

0

and

resistant

with

of dehydroas-

35

0

40

in vitro

treated

the effect

35

43

0

experiments

highly

of dehyand the

30

0

ascites

in animals

we tested

B- 12 on the

efficacy activity

on

30

32

B-12

B-l2,

B-12

buffered to pH 5.2 with 0.01 g sodium 1 mL sterile water. L12 10 leukemia

34

0

and

survival

plus

the mitotic

and

34

0

34

acid

indicating tumor

acid

36

32

0

ofdehydroascorbic

leukemia

of previous work acid in suppressing

to generalized F:/fect

or ascorbic

35

0

a sampling

results

acid L1210

41

C

Reference

Because droascorbic

bearing

As before, the vitamin mixture form, 0.01 g hydroxycobalamin,

T

represents

ofmice

C

C

group

12635

SURVIVAL

ofdehydroascorbic

survival

Citric acid, 85.6 mg/kg (pH 2.4) Ehnlich carcinoma

T

T, test; C, control.

ON

Days

5

after

10

P 388

20

15

25

30

35

40

transpLant

FIG 1 . Survival of mice bearing P388 leukemia after treatment with dehydroasconbic acid and hydroxycobalamin (vitamin B-l2). One hundred mice were sampled, SO treated (#{149} - - - #{149}) and SO control (0 0). Test mice were inoculated once daily with 0.2 mL of the vitamin

mixture

volume

of saline.

for 8 successive + = study

days: terminated.

control

animals

received

an equal

POYDOCK

l264S

agents.

Cobalt

sodium

ascorbate,

as had 60

-J

4

50 Control

,

,

DHA-B,2

,

,

the

above. comn when

,

40

the

. 30

acetate

and

were

10 5

20

25

30

35

SURVIVAL

40

45

50

55

60

(days)

FIG 2. Survival ofmice bearing leukemia L12l0 after treatment with a mixture of dehydroascorbic acid and hydroxycobalamin (vitamin BI 2). Eighty mice were sampled, 40 treated and 40 control. Test mice were injected with 0.2 mL ip of the vitamin mixture on days 1-8, 1 1, and I 3. Control mice received an equal volume of a saline solution of the same pH and osmolarity.

sodium

ascorbate,

oxidized,

determined

that

see Appendix

Ei,ect

ofdehs’droascor/nc

()ftt’()

solid

our

stock

ofascorbic

acid

had

vent

the

there

acid

and

B- 12 on growl/i

conducted

the C-B-12

B- 1 2, and

are rarely found in clinical situations, ofthe vitamin mixture on the growth

(II) ascorbate

that

agent. than

This

series

x 106) washed

that the vitamin of dehydroascorbic

(When

an ascites

is injected subcutaneously it develops into a solid tumor.) mice received Krebs 2 carcinoma and 50 mice received

Ehrlich

carcinoma.

The

and control. Starting received, in the area mg/kg

body

groups

were

24 h after ofthe tumor

wt) ofthc

then

ered to pH 6.6 with sodium an equal volume of Hank’s

randomized

tumor implantation, implant, 6 mg/0.2

dehydroascorbic

acid-B-l2

ascorbate. solution.

Control Treatment

transplantation,

tumors

to test test mice mL sc (240

mixture

buff-

animals received continued for

days

in three

after

dimensions

0.05-mm

in

treated animals than

tumor with

increments.

a metric Results

mice developed tumors, developed tumors, and in control

animals.

the

C/ze,nical

balt

growth

These

i?zt’(s’tigalions

Hydroxycobalamin atom bonded

conducted or the mixture

oft/fl’

is critical

whether

ascorbate

5. All un-

ofthe much

vitamin almost

treated smaller that

the

to the

at its center Experiments

carcinoma on mitotic

as are the

the

similar

and P388 leukemia index was examined

to those tumors after

ascorbate,

and

reduced

of cobalt-carbon that

that

that

of the basic some sort

presence to the

described were used, treatment

Bof

it is this complex

cobalt

demonstrated

(II) is much

cobalt

mixture acid

that

mixture

of mice The standing

was

first two the

with of these

results

cobalt

of the

(II) sulfate;

improves

that

efficacy

(un-

above.

Ehrlich

and the effect with various

Downloaded from https://academic.oup.com/ajcn/article-abstract/54/6/1261S/4715221 by guest on 02 April 2018

= 25.

the effect

dehydroascorbic acid that was effective. ofan effects

agent

on mitotic

on the

survival

tumors.

characteristics studies

may of the

0. 13 0.00

Reference

5.

t I ± SD. Controls all developed tumors. Mean of only two tumors that developed.

§ No

tumors

developed.

may

1) It was the

research.

ofagents, simultaneously,

those

survival

acid, which was effective in minot ascorbic acid itself. 2) It

Test

Knebs 2 Ehnlich

vitamin

of

dehydroascorbate-hy-

of subsequent

of other

Tumor

of the cobalt

of

mitotic

the activity is ineffective,

characteristics

for further

we used,

predictor

implanted

the

has several

directions

models

a good

and

dramatically

oxidized form, dehydroascorbic totic index and survival studies,

index

inhibiting alone

(II) acetate

demonstrated

and

a mixture inhibits

with the use of ascorbic acid instead is much less effective. Most important,

vitamin

clues

greatly

types without hydroxycobalamin

of tumor-bearing mice. This series ofexperiments provide

that

hydroxycobalamin

salts,

experiments

a

were

cobalt

droxycobalamin

a cowere

data). methods

cxshowed

be useful role

in under-

of ascorbate

TABLE 5 Size (cm2) of two types of solid tumor measured 1 wk after treatment with a mixture of hydnoxycobalamin and dehydroasconbic acid buffered to pH 6.6 with sodium ascorbate*

efficacy

is critical

to pre-

B-l2, when completely

mixture

it is the

of several fibroblasts;

3) In the tumor

measurements

demonstrate

B- 12) contains ring structure.

ring

measured

tumors.

vitamin

whether

and

results

solid

(vitamin in a comn that

in Table

acid and implantation,

to determine comn

published The

of these

with

are

whereas only two those tumors were

combination of dehydroascorbic injected at the site of tumor inhibits

were

dial caliper shown

B-12 large

OH,

as preservation to postulate notable

after

the The

sufficient

evidence

is formed,

appeared to be the combination and hydroxycobalamin, given

8 successive days. Ten

unreacted

It is also

that

Fractionation

Columbus,

showing

of experiments tumor that

solution.

Labs,

be-

suggested is effective.

B-l2.

activity normal

in Hanks

was

to Co(III).

contained

complex

is the active

differences

confirmed

present

covalent bond formation as well 1 2 structure. It seems reasonable

of solid tumors (5). Two transplantable solid tumors, Krebs 2 and Ehrlich carcinoma, were used. Female HA/ICR Swiss mice were injected subcutaneously in the left flank with 0. 1 mL (2 cells

reported

and sodium ascorbate to the reduced form.

Co(II)

compound

and

ascites

laboratory

at Battelle

mixture

a new

no significant

of ascorbate of the

dehydroascorbate

tumor Fifty

activity

Discussion

lutnors

Because ascites tumors we investigated the efficacy

excess

periments

were

in our

reoxidation

less expensive

A.)

with

mitotic

combination

dehydroascorbate from the oxidized

stoichiometric

cobalt we subsequently

combined

treated tubes. These experiments of cobalt and ascorbate that

experiments

mixture with was changed

that

when

in inhibiting

hydroxycobalamin-ascorbate

Further 10

sulfate,

Thus, it would appear that it is the cobalt and not the ring that is critical in the hydroxycobalamin. However, cobalt acetate and cobalt sulfate were used alone, without

tween the control and that it is a compound

20

cobalt

as effective

Controlt 1.63 ± 0.47 2. 19 ± 0.58

in

COMBINED relation

to cancer.

form, ascorbic effective

Some

in inhibiting

and

was

and

animal

investigators

have

acid; in our experiments mitoses

ineffective

only

we used.

used

just

the

C AND

when

given

survival

Although

at very in the

other

high

types

investigators

when

it is notable either

used

that

research

intraperitoneally seem that future

would of various

have

Others inhibitor reported

with

involved

or directly

to simple

in the

administration

acid.

presence

Finally,

of the

area

in food

Moreover,

of ascorbate,

Hogenkamp

of the

agents

tumor.

It

or water.

metals to the

(which

formation

antitumor

activity.

to warrant

further

Such

(9) reported

B- 12 undergoes

to the release ofthe cobalt nucleus. Hollis the C2 carbon atom of vitamin C served leading

agents.

have suggested that dehydroascorbate is an important ofcell growth and division (6, 7), and Omura et al (8) a 68% inhibition of growth of solid sarcoma 180 by

dehydroascorbic

transitional

other injection

research might examine the effectiveness of ascorbate when administered in that way,

forms

in addition

in the

in combination

our

include

both

reactions

of platinum-ascorbate

mechanisms

investigation.

that, leading

et al (10) showed that as a binding site for platinum

and results

and

cobalt),

that

Reikert acid

The

in vitro

Downloaded from https://academic.oup.com/ajcn/article-abstract/54/6/1261S/4715221 by guest on 02 April 2018

and

animal

5 were

reported

L-ascorbic

acid.

However,

that

with the

itself. the

active

the

active

oxidized 1. Poydock ME, Fandon JC, Gallina D, Ferro V, Heher C. Inhibiting effect ofvitamins C and Bl2 on the mitotic activity ofascites tumors. Exp Cell Biol l979;47:2l0-7.

L. Inhibiting

in ascites

tumors

effect

in mice.

of Exp

A

1, 3, and oratory

References

J, Aleandri

l985;l07:274-6.

Soc

appear B

D, Rice

on cell division

Cell Biol l982;50:34-8. 3. Poydock ME, Reikert D, Rice J. Influence of vitamins C and B12 on the survival rate of mice bearing ascites tumor. Exp Cell Biol 1982;50:88-9 1. 4. Poydock ME, Harguindey 5, Hart T, Takita H, Kelly D. Mitogenic inhibition and effect on survival of mice bearing L12l0 leukemia using a combination ofdehydroascorbic acid and hydroxycobalamin. Am J Clin Oncol 1985;8:266-9. 5. Poydock ME, Phillips L, Schmitt P. Growth-inhibiting effect of hydnoxycobalamin and L-ascorbic acid on two solid tumors in mice. IRCS J Med Sci 1984;12:8l3. 6. Edgar JA. Is dehydroasconbic acid an inhibitor in the regulation of cell division in plants and animals? Experientia l969;25:1214-5. 7. Mills CA. Bone marrow nutrition in relation to the phagocytic activity ofblood granulocytes. Effect ofasconbic acid on phagocytic activity established quantitatively. Blood l949;4:l53-9. 8. Omuna H, Tomita Y, Yasuhiko N, Murakami H. Antitumon potentiality of some ascorbate derivatives. J Fac Agric Kyusu Univ l974;l8: 18 1-9. 9. Hogenkamp HPC. The interaction between vitamin B-12 and vitamin C. Am J Clin Nutr l980;33:l-3. 10. Hollis LS, Amundsen AR, Stern EW. Synthesis, structure and antitumon properties ofplatinum complexes ofvitamin C. J Am Chem

APPENDIX

displayed

would

ME,

dehydroasconbic

doses

tumor

12655

SURVIVAL

ON

2. Poydock

reduced

found ascorbic acid to be effective in various animal tumor models, future research should be careful to distinguish between these two forms and to examine their effectiveness separately. Furthermore, relatively few investigations have been carried out with combinations of ascorbate and other agents. Our results suggest that future research should examine the effectiveness of ascorbate

B-l2

that form was moderately

in prolonging

models

VITAMINS

The present

agent

in the

earlier

paper

used has

therefore

as dehydroascorbic

in references

papers

to have

experiments

purchased

dehydroascorbic

terminology

reported

published

subsequent

use of freshly agent

form,

experiments in the

ascorbic

work acid,

in the

than

lab-

revealed been

the

ascorbic of that

corrected

rather

acid

probably than

descriptions

been

acid

had rather

used

in our

acid work

in

to refer

to the

ascorbic

acid.

Over 200 Alternative Cancer Treatments Plus Other Information Written by Webster Kehr, Independent Cancer Research Foundation, Inc. | Last updated on February 22, 2015 | Filed under: Articles  Prev Article

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Notice: This page is NOT designed to be a tool for a person putting together an alternative treatment for cancer, except perhaps to look for a “base” treatment. I have a tutorial and other articles that are designed to do that! This page is only intended to be a reference to demonstrate the massive amount of scientific evidence for the use of natural substances in cancer treatments. This list does not include key warnings, dosages, etc. Click on this CancerTutor Home Page link if you are putting together an alternative cancer treatment program. CancerTutor Home Page Introduction The FDA and AMA would like you to believe there is “no scientific evidence” that natural substances are useful in treating cancer. That in spite of the fact that pharmaceutical companies routinely use natural substances to inspire the design of their synthetic toxins. A casual reading of this page will demonstrate that natural substances are the preferred way of treating cancer. It makes one wonder why the National Institutes of Health have ignored Mother Nature (of course we know why they have been ignoring scientific evidence). I have put together a listing of cancer treatments, special foods, special nutrients, etc. that have either been proven to treat cancer or have demonstrated significant potential to treat cancer. I have put two asterisks (**) by treatments that are definitely in the “top 50” of alternative cancer treatments. A single asterisk (*) means this treatment deserves serious consideration. Just because a treatment does not have an asterisk does not necessarily mean it is a weak treatment, it may mean I haven’t had time to research the substance yet or the evidence for the product is still being investigated. =======================================================

The List ======================================================= 714­X or 714X / Gaston Naessens Homeopathic remedy out of Canada. Good results if the cancer is caught early. Mediocre results if

the cancer is caught in a late stage. See my detailed article linked to on my main page Abscisic Acid See Livingston­Wheeler Active Hexose Correlated Compound (AHCC) “Stimulating NK cell function and supporting the immune system’s front­line defense has become one of the most promising avenues of cancer treatment and prevention. Since 1986, 29 published scientific studies using a proprietary natural compound called AHCC (Active Hexose Correlated Compound) made from a hybrid of mushrooms used in traditional Japanese medicine, has been shown to increase NK cell cancer killing function by several hundred times, providing benefits for the majority of cancer patients in several human clinical trials. Substantial improvement is also shown in the functioning of macrophages, another type of cytotoxic white blood cell that clears debris from cell damage, directly attacks disease causing cells, and displays antigens which stimulate antibody production by B­cell white blood cells, and in the production of specific cytokines, immune system chemical messengers…” Agaricus Blazel Murill (ABM) (Mushroom) “The polysaccharide contained in Agaricus Blazel Murill vitalizes production of interferon and interleukin in small animals (guinea pigs). This effect indirectly functions to destroy or prevent the proliferation of cancer cells. This refers to a cytokine inducing effect… Clinical results obtained in collaboration with university researchers and hospital since the report on the anti cancer effect of Agaricus Blazei Murril (ABM Mushroom) was released at the general convention of the Japan Cancer Association in 1980 proved that although many fungi polysaccharide only effect solid cancer and polysaccharide of Agaricus Blazei Murril is effective against Ehrlich’s ascites carcinoma sigmoid colonic cancer, ovarian cancer, breast cancer, lung cancer, and liver cancer as well as against solid cancer.” * Alfalfa (Medicago Sativa) “This plant contains large amounts of chlorophyll, beta­carotene, and vitamin E. It also contains the amino acid L­Canavanine, which has: antibacterial, antiviral, and anti­tumoral activity.” Algae (e.g. Blue­Green) See Spirulina * Aloe Vera Aloe Vera is generally used in conjunction with other treatment programs, such as Cesium Chloride. It contains multiple phytonutrients known to treat cancer. ** Alkalinity / Cesium Chloride / Calcium Chloride If cancer cells get too alkaline, they first stop spreading, and then they die. Also used to stop the pain of cancer very quickly. See my detailed article linked to on my main page

* Alpha Lipoic Acid (ALA) “Other research on alpha­lipoic acid has shown that it might: … inhibit the activation of “nuclear factor kappa­B,” a protein complex involved in cancer and the progression of AIDS. (Suzuki YJ, et al., Biochemical & Biophysical Research Communications, 1992;189:1709­15). ‘The therapeutic potential of alpha­lipoic acid is just beginning to be explored,” observed Packer, “but this compound holds great promise.'” [ALA is generally used for diabetes.] Alsihum (formerly: Alzium) “Eight of the plant extracts in Alsihum were studied extensively for cytotoxic effects on cancer cell lines at the Cancer Pharmacology Laboratory of Children’s Mercy Hospital in Kansas City, MO. The human cell lines tested included leukemia, colon, glioma, breast, ovarian, adrenal and lung cancer. The principal researchers were Dr. Albert Levya, PhD., Director of the Cancer Pharmacology Lab; and Dr. Arnold I. Freeman, M.D., the Chief of Hematology and Oncology. Our results indicate that the plant extracts in Alzium contain cytotoxic substances which have differential effects against these specific forms of tumor cells. Even cell lines over 100 times more resistant to conventional chemotherapy drugs were considerably sensitive to Alzium.” Anti­angiogenesis Therapy Orthodox Anticoagulants “Patients with cancer are more likely to develop blood clots (thrombosis) than normal individuals. Indeed, a blood clot can be the first indication that a cancer exists. This may be because many cancer cells contain a protein called Tissue Factor (TF) which can rapidly initiate blood clotting. This suggests that the blood clotting system is important in the ways cancers grow and spread (metastasize) in the body. This theory is supported by the fact that commonly used anticoagulant drugs such as heparin and coumadin have prolonged survival of patients with some forms of cancer.” * Antineoplastons – Burzynski Very expensive alternative treatment by an M.D. It is best known for its success at treating brain cancer. See my detailed article linked to on my main page Antioxidants A category, not a treatment. Many antioxidants are used as treatments for cancer, or as supplementary treatments for cancer (supplements as part of an overall alternative treatment plan. Anvirzel “Anvirzel is a patented extract from the Nerium Oleander, a common house plant. [The plant is very toxic, however] the extract (Anvirzel) has been the twenty year study of a Dr Ozel from Turkey… Reports that slipped out in late 1999 showed that Anvirzel reversed AIDS, no matter what the phase of the disease, arthritis, psoriasis, hepatitis C, and even diabetes in some cases.

Initially, Anvirzel was thought to work only on cancers found early, however, very positive results have been found in people given just weeks to live. To top this all off, Anvirzel seems to be the first cancer remedy to show positive results for leiomyosarcoma, probably the deadliest of cancers. Anvirzel also crosses the blood­brain barrier (like Poly­MVA) and gives hope to people with brain tumors.” Anvirzel is made by Ozelle Pharmaceuticals, Inc. Also, see the article below on: “Oleander Soup” * Arginine / L­Arginine (amino acid) “A positive study conducted by a team of German researchers showed that arginine contributed significantly to immune function by increasing levels of white blood cells. Scottish scientists added that dietary supplementation with arginine in breast cancer patients enhanced NK cell activity and lymphokine cytotoxicity (Brittenden et al. 1994). (Lymphokines are chemical factors produced and released by T­lymphocytes that attract macrophages to a site of infection or inflammation in preparation for attack.) Various researchers have shown that increasing arginine increases neutrophils (white blood cells that remove bacteria, cellular debris, and solid particles), significantly upgrading host defense (Muhling et al. 2002). Apart from enhancing immune function, arginine increases a number of amino acids, creating the possibility of an amino acid imbalance. Oversupplying some amino acids while undersupplying others is thought to destabilize the tumor. All cells, both healthy and diseased, have amino acid requirements; if not met, the cell is significantly handicapped (Muhling et al. 2002). Amino acid manipulation has been applied in oncology for decades with varying degrees of success.” See: http://www.lef.org/protocols/prtcl­027a.shtml Arlin J. Brown Treatment This treatment includes purple grape juice, but includes several other nutrients as well. Arnold Ehret Diet (Mucusless) This diet consists of water fasting and a type of raw food vegan diet, with an emphasis on certain fruits. Like all vegan diets, if using this diet for the treatment of cancer, there should be a strong emphasis on the allowable foods that are known to treat cancer. See: http://www.arnoldehret.org/ See the Raw Food section for more information. Artemisinin see Wormwood herb Aveloz “A spurge shrub native to South America may hold the key to victory in the battle to destroy cancer. Aveloz, known as mataverruga (“kill­wart”); one drop of its solution when applied to a wart has been known to destroy a growth in one day. That extraordinary property​ the power to eliminate ​ neoplasms, malignant (skin cancers) drew the attention of professors of medicine. Here was an adequate tumor remedy, near at hand. Why not try it INTERNALLY to eliminate cancerous tumors? They did and it worked. Five to ten drops of that same solution, depending on size and density, taken every hour, has been known to eliminate cancerous growths in one week. The hard tumor collapses. What happens is that the cancer has been reduced to an ill­smelling paste by the

tissue tearing (escharotic) properties of the solution. The elimination of the malignancy will cause an irritation of the kidneys, but that’s all. You can alleviate that side effect by taking extra Vitamin C. ” See: http://www.lifex.com/special.html Avocados “Avocados also exceed other fruits as a source of the potent antioxidant lutein, according to Susan Bowerman, R.D., a registered dietitian at the University of California at Los Angeles Center for Human Nutrition. Lutein may also safeguard your cardiovascular system from atherosclerosis (or hardening of the arteries) and prevent prostate cancer.” AyurVeda Medicine A general category referring to natural medicine practiced in India. “Ayurveda, literally, “the science of life,” is the ancient Indian art and science of healing and rejuvenation through the use of natural herbs, roots and minerals.” Beard Enzyme Therapy This treatment led to Metabolic therapy. “The Scottish embryologist, Dr. John Beard, proposed in 1906 that the pancreatic proteolytic enzymes represent the body’s main defense against cancer, and would be useful as a cancer treatment. Particularly during the first two decades of twentieth century, Dr. Beard’s thesis attracted some attention in academic circles, and several case reports in the medical literature documented tumor regression and even remission in terminal cancer patients treated with pancreatic enzymes. In 1911, Dr. Beard published a monograph entitled The Enzyme Therapy of Cancer, which summarized his therapy and the supporting evidence.” * Bee Pollen “Do not give honey or any other bee food to an infant under one year of age. Honey contains dangerous spores that an infant’s immature immune system cannot fight. These spores are not a problem for the immune systems of older, healthy children and adults.” “In his summary, Dr Robinson reveals the incredible results: “In the mice not given bee pollen, mammary tumors appeared at an average of 31.3 weeks. Tumor incidence was 100% and they all died”. “The average onset of tumors of the mice given bee pollen was 41.1 weeks… around 30% later in time”. “Seven mice in the bee pollen group had still not developed a tumor at 62 weeks of age when the tests were terminated”. Dr Robinson concluded that bee pollen contains an anti carcinogenic principle that could be added to food.” * Bee Propolis “The latest FDA­approved drug for fighting colon cancer [Celecoxib] may be sitting in your kitchen. Rosemary, turmeric, grapes, a honey bee product called propolis: These all contain chemicals similar to a drug used to prevent the growth of cancerous cells in the large intestine; they work much in the same way.” It is interesting that the FDA approved the drug, but not the natural substances that contained a natural version of the synthetic drug. See the book: Beyond Aspirin: Nature’s Answer to Arthritis, Cancer & Alzheimer’s Disease (Hohm Press, 2000), by Thomas

M. Newmark and Paul Schulick Bee Venom “Melittin is the main active component of bee venom. Percentage level of its contents in bee venom is 50­60%. Melittin provides very strong anti­inflammatory and anti­bacterial effect. Scienticts at Australia’s Commonwealth Scientific and Industrial Research Organization of Molecular Science use melittin to develop cancer treatments that should have fewer side effects than other drugs used to fight the disease.” * Beet Juice (Kills cancer cells) A common addition to vegetable juices used to treat cancer. “Beet juice is used in Europe for the treatment of cancer,” says Eleonaore Blaurock­Busch, Ph.D., president of Trace Minerals International in Boulder, Colorado. The crimson­like pigment in beets, betacyanin, is the compound believed to have the anti­cancer properties. Compared to other juices used in studies, beet juice ranked close to the top in preventing cell mutations that commonly lead to cancer. Beets are also high in folate, which is a pregnant woman’s favorite mineral for prevention of birth defects.” “Never drink beet juice by itself. Beet juice should always be mixed with other vegetables and/or apple juice. Pure beet juice (from the bulb or greens) can temporarily paralyze your vocal chords, make you break out in hives, increase your heart rate, cause chills or a fever.” See also the Raw Food diet. ** Beetroot (Betaine, OPCs) “The Hungarian Professor Bakay of the University of Budapest carried out experiments in 1939 (long before Dr Ferencz) on 72 patients suffering from cancer or leukemia in his clinic in the Hungarian capital. He observed regression of the tumors, increases in weight and improvement in the general condition of his patients.” “Dr. Ferenczi’s clinical report included methods of administering the beets and several very important case studies: ‘I diagnosed a man of 50 years of age, with a lung tumour. And subsequently confirmed in a Budapest hospital and also in a country hospital, which corresponded clinically to lung cancer. I started treatment with beetroot in the described manner. After 6 weeks of treatment the tumor had disappeared … after 4 months of treatment he gained 10 kg. In weight, the erythrocyte [mature red blood cell] sediment rate [e.s.r] was reduced drastically. Thus he represented the symptoms of a clinical recovery'”. See also: Raw Foods Beres Drops (Dr. Jozsef Beres) “In Hungary he carried out tests on 235 patients who had ‘no chance of recovery.’ After giving them the drops nearly ONE THIRD found their tumours had subsided.” ** Beta Carotene A carotenoid especially effective in killing leukemia cells. In some cases it offsets the benefits of Vitamin E (and vice versa). See also: Raw Foods * Beta Glucan “Glucan is a potent reticuloendothelial­modulating agent whose immunobiological activity is

mediated, in part, by an increase in the number and function of macrophages.” “There is now evidence to show that beta glucan may be the most ubiquitous macrophage activator in nature. Once activated the macrophage, through its ability to initiate a cascade of cellular responses, becomes the central conductor of the immune symphony. A macrophage can recognize and kill tumor cells nonspecifically, as well as remove foreign debris.” The purest, most potent form of Beta Glucan is the Transfer Point Brand. Call800.746.7640 for more information on doubling your immune system. Bitter Melon (e.g. Chinese Bitter Melon) “Bitter Melon stimulates the release of insulin and blocks the formation of glucose in the bloodstream, which may be helpful in the treatment of diabetes, psoriasis, cancer, infections, and pain from neurological complications, and may delay the onset of cataracts or retinopathy and inhibit the AIDS virus.” There has not been a lot of scientific work on Bitter Melon, so its exact benefit for cancer patients is still unknown. * Black Seed Oil / Black Cumin / Nigella Sativa (herb) “These results confirmed earlier findings that Black seed has a positive stimulating effect on the immune system. These findings are of great practical significance since they indicate that a natural immune enhancer like the Black seed could play an important role in the treatment of cancer, AIDS, and other disease conditions associated with immune deficiency states.” Boluses “A bolus is like a suppository, used either vaginally or anally. Dr Schulze created a bolus of Squaw Vine herb, Slippery Elm Bark, Goldenseal root, Yellow Dock root, Comfrey root, Marshmallow root, Chickweed herb, Mullein leaf, Garlic bulb and Coconut and Tea Tree oil. Some boluses can be made into suppositories by mixing the materials and placing them into the freezer. These would be used for cervical problems.” Bloodroot Bloodroot is a key ingredient, along with zinc chloride, in almost all topical salves used in the treatment of skin cancer. For more than a hundred years, bloodroot has been successfully used to treat skin cancers. However, regardless of what some vendors claim, bloodroot should not be taken internally, not even in small doses. “Researchers have isolated the alkaloidal principle, sanguinarine, as the anticancer constituent. This alkaloid is also found in the far more abundant greater celandine, Chelidonium majus, an herb common along roadsides in Europe and elsewhere.” See also Cansema Boron Neutron Capture Therapy (Brain Cancer) Orthodox – “If your tumour is a Glioma, you should investigate BNCT, – boron neutron capture therapy – a one­session radiotherapy treatment, with minimal side­effects, and improved survival to date. This promising technique is still in trials.” Boswellic Acids “Boswellic acids are the compounds isolated from the gum resin of Boswellia serrata and have

been used for the treatment of inflammatory diseases for many years in the countries of the east. Recently, a few studies showed that the acids may have anti­cancer effect on leukemia and brain tumors. We investigated the apoptotic and anti­proliferative effects of two types of boswellic acids, keto­beta­boswellic acid and acetyl­keto­beta­boswellic acid, on liver cancer Hep G2 cells … The acids may be a promising drug for the chemo­prevention of liver cancer.” Bovine Cartilage (the Good and the Bad) “The first published clinical results of the effectiveness of bovine cartilage in the treatment of cancer appeared in 1985, after a successful five­year trial under FDA permit on 31 terminal patients who had been given the full possible spectrum of orthodox treatments and had failed to respond. The results were encouraging. “Of the 31 patients cited, 90 percent showed a partial or complete response. Eleven (35 percent) showed complete response with probable or possible cures, eight patients (26 per cent) had a complete response but sustained a relapse; six sustained a partial response. Of the 35 percent who showed complete response, nine lived on without any cancer activity and died of non­cancer related diseases or old age.” Although all types of cartilage are a rich source of the active ingredients for healing, the cartilage type appears to influence the chances of recovery. Research has shown that although shark cartilage contained 1000 times more of the active ingredients, their release from shark cartilage took 41 days in vitro. It would appear that this difficulty is the reason why such large amounts are necessary in the use of shark cartilage, whereas all the successful bovine results have been obtained using only 9 grams per day.” Having said all of that, the respected Minnesota Wellness folks question these studies: “This is much cheaper than Shark Cartilage and is being presently studied here in America with, ostensibly, very good results. However, the studies have been fudged, the success rate of 99% is highly inflated, and the people conducting these studies [I am not sure who he is talking about] seem to lack even quintessential ethics. Thus we cannot recommend bovine cartilage to anyone.” ** Brandt Grape Cure Purple grapes, with their seeds and skin, contain more than a dozen nutrients known to kill cancer cells. They are especially effective when used in conjunction with a 12 hour fast. The Johanna Brandt Grape Cure should not be combined with any other alternative cancer treatment without carefully considering whether it will neutralize the grape cure. See my detailed article linked to on my main page ** Breuss Total Cancer Treatment This 42 day special fast (which includes a special tea) literally starves cancer cells to death (the tea is very low in glucose). See my detailed article linked to on my main page ** Budwig Diet , Flaxseed oil + Cottage Cheese This is a superb treatment, however, it is critical to follow ALL of the advice of Johanna Budwig. When done properly, almost every cancer treatment known to mankind would be helped by adding this treatment to it. It is easy to follow and inexpensive.

See my detailed article linked to on my main page * Burdock Root (herb) “The burdock root is comprised mainly of carbohydrates, largely INULIN (not insulin), mucilage, starches and some sugar. Inulin is the principle active ingredient in burdock root and it has been shown to have remarkable curative powers in lab studies. Inulin helps strengthen the organs, especially the liver, and its natural sugars help to regulate blood sugar metabolism. Some diabetics claim that they have been able to eliminate the need for taking insulin … Inulin is also regarded as a powerful immune system regulator, and when teamed up with echinaceait is a powerful immune system booster. Inulin is thought to attach itself to the surface of white blood cells and make them work better. It is even thought that Inulin can activate T­Cells in the attack against cancer cells.” See also Essiac Tea Butyrate (CAM) “A study published in the January 2000 issue of Carcinogenesis shows that human colorectal cancer cells can be made more sensitive to butyrate (a European cancer therapy) when the butyrate is combined with a COX­2­inhibiting drug. Butyrate helps to induce the differentiation and death (apoptosis) of colorectal tumor cells, but is not readily available in the United States. The doctors conducting this study stated that dietary modification (using therapies such as butyrate) along with COX­2­inhibiting drugs could be considered in the treatment of colon cancer.” Caffeic Acid Phenethyl Ester (CAPE) See Bee Propolis Cancell see Protocel Cannabinol (CBN) (yes, medical marijuana) See: Tetrahydrocannabinol ** Cansema / Black Salve (Skin Cancer) The elements of this treatment have the longest proven track­record of any cancer treatment. It is so effective the FDA recently shut down the largest seller of this product. See my detailed article linked to on my main page Cantron see Protocel Carnivora — Venus Flytrap “For almost three decades, world renowned oncological pioneer Dr. Helmut Keller has developed powerful non­toxic synergistic treatments for the presence of life threatening diseases — as well as chronic infectious and other auto immune diseases and disorders. In 1973 he came upon the dionaea muscipula species of natural plant. His discovery would yield a patented extract he would call “Carnivora.” Little did he know that some 30 years later Dr. Keller and his Carnivora would be responsible for having saved and prolonged many lives around the world from the ravages of one

of the world’s most deadly diseases — Cancer.” ** Carrots “In a review of 206 human studies, carrots consistently emerged as one of the top cancer­fighting foods. The power of carrots lies in the group of pigments called carotenoids (beta­carotene is among this group), which give them their orange color.” See also: Raw Food Diet Castor Oil Packs “A double­blind study, described by Harvey Grady in a report entitled Immunomodulation through Castor Oil Packs published in a recent issue of the Journal of Naturopathic Medicine, examined lymphocyte values of 36 healthy subjects before and after topical castor oil application. This study identified castor oil as an anti­toxin, and as having impact on the lymphatic system, enhancing immunological function. The study found that castor oil pack therapy of a minimal two­ hour duration produced an increase in the number of T­11 cells within a 24­hour period following treatment, with a concomitant increase in the number of total lymphocytes. This T­11 cell increase represents a general boost in the body’s specific defense status, since lymphocytes actively defend the health of the body by forming antibodies against pathogens and their toxins. T­cells identify and kill viruses, fungi, bacteria, and cancer cells.” * Cat’s Claw (herb) (Uncaria Tornentosa) “Peruvian Dr.s have reported good success in treating hundreds of Cancer patients.” “In addition, Cat’s Claw tantalizes scientists with it’s anti­cancer actions. In the laboratory, it kills cancer cells and stops them from reproducing. When given to people with cancer, it repairs DNA damage, a vital first step, in cancer protection.” * Cayenne Pepper “Hot chili peppers not only fire up your food, they may also put the heat on cancer cells and force them to self­destruct. A new study shows a natural substance found in chili peppers kills cancer cells by starving them of oxygen. Researchers tested the chili pepper substance (known as capsaicin) along with a related compound (resiniferatoxin) on human skin cancer cells to analyze how the cells reacted. Both compounds are natural substances known as vanilloids. They found that the majority of the skin cancer cells exposed to the substances died. The researchers say these substances seem to kill cells by damaging the cell membranes and limiting the amount of oxygen that reaches the cancer cells.” * Chaparral (herb, toxic – stay within dosages) This herb has a long history of success in treating cancer. The FDA has shut it down as a treatment for cancer. Congress allows 400,000 Americans a year to die from tobacco related products, but the FDA doesn’t allow you to treat your cancer with chaparral. * Cherries “[Cherries] are a top source of perillyl alcohol, which kills cancer cells but spares healthy cells. In animal studies, this substance shrank pancreatic, breast, and liver tumors.” Chicory Root

“Chicory root, a popular ingredient in herbal coffees, contains an anti­cancer carbohydrate known as inulin. Inulin prevented the formation of colon cancer tumors in several animal studies, according to a review published last year in the British Journal of Nutrition.” * Clodronate This treatment stops breast cancer from spreading to bone cancer. Not available in America. ” The Food and Drug Administration’s suppression of clodronate may have caused the premature or needless death of about 30,000 American women each year, based on the Aug. 6, 1998, New England Journal of Medicine study. Since clodronate could have been made available 15 years ago, about 517,000 American breast cancer victims were forced to suffer agonizing bone metastasis, and a total of 450,000 women probably died prematurely because the FDA aggressively denied this drug to cancer patients.” “In 1998, the New England Journal of Medicine published impressive data indicating that clodronate reduced the incidence and number of metastasis in bone and viscera (organs enclosed in the abdominal, thoracic, or pelvic cavity) in high­risk breast cancer patients by 50% (Diel et al. 1998; also see Journal Club on the Web).” See: http://www.mnwelldir.org/docs/cancer1/altthrpy.htm ** Coenzyme Q10 Amazing antioxidant that treats cancer. It is part of the well­known Stockholm Protocol. See my detailed article linked to on my main page ** Colloidal Silver This is one of several colloidal mineral drinks that show promise for cancer treatments. “Naturopathic Medicine regards Cancer as a viral and fungal [candida septicemia] process. Microorganisms depend on a specific enzyme to breathe. Colloidal Silver is a catalyst that disables these enzymes, and as a result they die. To this day, there has been no recorded case of adverse effects from it when it is properly prepared. There also has been no recorded case of drug interaction with any other medication. Unlike pharmaceutical antibiotics which destroy beneficial enzymes, Colloidal Silver leaves the tissue­cell enzymes intact.” Colonel Joe (Oxalic Acid) The Colonel Joe protocol is headed by carrot juice. He claims it is the oxalic acid in the diet that kills the cancer cells. I personally wonder whether the success of this treatment is because of the beta carotene or the oxalic acid in carrots. See: http://www.coljoe.com/ Colostrum This is a product of a mother’s natural milk. ” Steven Rosenberg’s 1985 book, Quiet Strides in the War on Cancer, first popularized the benefits of cytokines in the treatment of cancer. Since then, the same cytokines found in colostrum (interleukins­1,­6 and ­10, interferon gamma and lymphokines) have been the single most researched factors in cancer research. Lactalbumin, also found in colostrum, has been found to cause the selective death of cancer cells, leaving the surrounding noncancerous tissues unaffected. And finally, Lactoferrin has similarly been reported to possess anti­cancer activity. And, as in heart disease, if viruses are responsible for either the

initiation or the spread of cancer, colostrum could be one additional way to prevent the disease.” Comfrey plant (Symphytum officinale) (H. E. Kirschner, M.D.) “Dr. Kirschner personally observed the powerful anticancer effects of comfrey on a patient of his who was dying from advanced, externalized cancer. He prescribed fresh, crushed­leaf comfrey poultices throughout the day. He writes that, “Much to the surprise of the patient and her family,” there was obvious healing within the first two days of treatment, with continued visible improvement over the next few weeks. “What is more,” he writes, “much of the dreadful pain that usually accompanies the advanced stages of cancer disappeared,” and there was a dramatic decrease in swelling.” The leaves should only be used EXTERNALLY. The medical community has greatly persecuted this plant. Their evidence is probably created by the misuse of the leaves. Contortrostatin “Contortrostatin is a disintegrin purified from southern copperhead snake venom. Disintegrins are small, disulfide­rich proteins containing an R/KGD (Arg/Lys­Gly­Asp) sequence at the tip of a flexible loop protruding from the main polypeptide chain. Integrins are a family of cell surface proteins found on many cell types that mediate interactions between cells, and between cells and their surroundings. Contortrostatin binds to integrins on the surface of cancer cells and inhibits tumor growth and metastasis. Contortrostatin is unique from all other disintegrins described to date in that it is a homodimer, which means it has two identical peptide chains held together by covalent disulfide bonds. We have been investigating the anti­tumor activity of contortrostatin using a breast cancer model. Our findings indicate that contortrostatin blocks several critical steps in tumor metastasis, and is, therefore, more potent than other agents which only block a single step. In addition, contortrostatin significantly inhibits invasion of breast cancer cells through an artificial barrier similar to the tissue surrounding blood vessels. This action was most likely due to the ability of contortrostatin to inhibit cell motility.” * Copper One of the colloidal minerals that has great potential for treating cancer. “In 1930, work in France indicated that injections of colloidal copper mobilized and expelled tumor tissue. Recent work with mice in the U.S. has shown that treatment of solid tumors with non­toxic doses of various organic complexes of copper markedly decreased tumor growth and metastasis and thus increased survival rate. These copper complexes did not kill cancer cells but caused them to revert to normal cells. Based on work in the treatment of cancers using copper complexes, researchers have found that these same complexes may prevent or retard the development of cancers in mice under conditions where cancers are expected to be induced.” Coriolus Versicolor (Mushroom) “PSK [Coriolus Versicolor] acts as an immuno­modulator and is used primarily in conjunction with chemotherapy, radiation, and surgical treatments for cancer. Clinical studies have demonstrated significant results, results that would make headlines if obtained through conventional treatments: 30% vs. 10% disease­free survival for colon cancer patients over and eight­year clinical trial when PSK was used alone and tested against a placebo; 22% vs. 5% survival at five years for stage III

lung cancer patients who were given radiation plus PSK as opposed to radiation alone; 81% vs. 64% survival at ten years for breast cancer patients who were given chemotherapy plus PSK as opposed to chemotherapy alone; 73% vs. 60% survival at five years for gastric cancer patients who combined daily PSK use with their chemotherapy as opposed to chemotherapy alone. This study, published in Lancet, found these results to be significant.” While PSK may have a profoundly higher survival rate than orthodox treatments, it is definitely not in the “top 50” of alternative treatments as currently used. C­Statin “C­Statin is a group of proteoglycan molecules (PGM) isolated from Bindweed (Convulvulus arvensis,) a common garden weed. C­Statin is an anti­angiogenesis product. No toxicity has been noted at normal doses. Patients with cardiovascular disease should use caution and, as with cancer patients, only use this product with a physician’s supervision.” Cranberry Juice “Researchers at The University of Western Ontario have completed a study that suggests cranberry products could have cancer­fighting properties. The study, funded by Ocean Spray Cranberries, Inc, documented that regular consumption of cranberry products may inhibit the development of breast cancer tumors in animals. Cranberries are a rich source of flavonoids, a variety of compounds produced by plants, that have been investigated for their anti­cancer activity.” ** Curcumin / Turmeric “Imagine a natural substance so smart it can tell the difference between a cancer cell and a normal cell; so powerful it can stop chemicals in their tracks; and so strong it can enable DNA to walk away from lethal doses of radiation virtually unscathed. Curcumin has powers against cancer so beneficial that drug companies are rushing to make drug versions. Curcumin is all this and more. Curcuma longa is a ginger­like plant that grows in tropical regions. The roots contain a bright yellow substance (turmeric) that contains curcumin and other curcuminoids. Turmeric has been used in Ayurvedic and Chinese medicine for centuries. But it’s only within the past few years that the extraordinary actions of curcumin against cancer have been scientifically documented. Among its many benefits, curcumin has at least a dozen separate ways of interfering with cancer.” See”http://www.lef.org/magazine/mag2002/jul2002_report_curcumin_01.htmlHere is another case where the drug companies are trying to synthetically modify a natural substance which is perfectly capable ofcuring cancer. Cytoluminescent Therapy (CLT) “CLT is an advanced form of photodynamic therapy, which uses light to fight cancer. Of course, light by itself cannot kill cancer cells. However, if patients are pre­ treated with a drug called a sensitizer, and are then administered light of a certain wavelength, their cancer cells will die by the millions. This is because the energy of the beam of light sets off a “bomb” within the cell. The “bomb” in this case is singlet oxygen, a kind of free radical. This process does not harm normal

cells because the sensitizing drug accumulates only in cancer and other abnormal tissues. Dandelion Plant (herb – NOT just the root) Yes, this is the plant that infests your yard. “Dandelion contains high levels of potassium, is a rich source of iron and vitamins, and, ounce for ounce, contains more carotene than carrots.” “Dandelion greens contain 7,000 units of Vitamin A per ounce. This is so high one author said it should make a carrot blush! It is important to realize that there is always a vitamin A deficiency in a person found to have cancer. The Chinese have used Dandelion for breast cancer for over a thousand years. Inulin, one of the major chemicals in Dandelion, is currently being studied extensively for its immuno­stimulatory functions. In testing it against cancer, it has been shown to be active against two tumor systems, by stimulating the actions of the white cells.” Actually, the list of cancer­fighting nutrients in dandelions is quite long. ” In l979, Japanese researchers found a dandelion extract – since then patented [I assume it was the extraction process that was patented] – which inhibits Erlich ascites cancer cells.” * DHEA (Dehydroepiandrosterone) “DHEA is a natural sterone produced by the adrenal gland. DHEA is the most common sterone in human blood, but amounts decline rapidly with age. Secretions are highest during the early twenties and begin to decline at around age 25, by the time we reach 70 years of age, DHEA production is only a small fraction of what it was 50 years earlier. Research has shown a correlation between low DHEA levels and a declining immune system, and DHEA is being used in the fight against HIV, cancer and senile dementia. It is also been clinically shown that DHEA helps brain neurons establish contact. Further, it is known that Alzheimer patients have low DHEA levels, when compared to their healthy counterparts. It is also known that a small amount of sulphate DHEA and micronized DHEA can convert into testosterone [which is a treatment for prostate cancer].” Diethylstilbestrol (DES) This drug is a synthetic hormone that was found to be in an alternative treatment for prostate cancer manufactured in China called PC­SPES. This drug was not supposed to be in the formula (along with two other manufactured drugs found in the formula). The DES can cause blood clots. Some doctors think the DES was the element of PC­SPES that made it work so well on advanced prostate cancer patients. One of the other manufactured drugs found in PC­SPES was a blood thinner. While PC­SPES has been removed from the market (I do not know if it is back on the market), doctors are now looking at DES as a treatment for advanced prostate cancer. * D­Glucarate (phytonutrient) “When a carcinogen known to induce intestinal cancer was given to rats, D­glucarate was shown to inhibit adenocarcinoma formation when given at the initiation stage. When administered after tumor development, D­glucarate significantly inhibited the size and metastatic potential of intestinal and colon cancers. The researchers made comments suggesting that D­glucurate may be effective in the prevention and treatment of cancer by inhibiting the beta­glucuronidase enzyme

and by inhibiting cancer cell proliferation induced by chemical carcinogens.” * DIM (diindolylmethane) DIM is a phytochemical that is found in broccoli, cabbage, turnip and mustard greens, kale, brussel sprouts, collards, etc. “The first development in this research using chemically altered [sic] DIM from broccoli came when the growth of breast cancer cells was inhibited in laboratory studies. Subsequent research showed these compounds also inhibited growth of pancreatic, colon, bladder and ovarian cancer cells in culture, Safe said. Limited trials on lab mice and rats have produced the similar results, he noted.” “Researchers from the University of California at Berkeley looked at the effects of broccoli on human breast cancer cells. According to findings, compounds in broccoli known as indoles are digested and broken down in the stomach to a compound called 3,3′­diindolylmethane (DIM). This compound may be the key to keeping cancer at bay.” * D­limonene “Cancer prevention, inhibition, and regression are the most note worthy attributes of the MTs [Monoterpenes]. D­limonene (DL) and perillyl alcohol (POH) have been shown to be chemopreventive against mammary, liver, lung, UV­induced skin cancer and chemotherapeutic against both experimental mammary and pancreatic tumours. Perillyl alcohol stands out as effective against human pancreatic cancer, colon, liver to reduce vein graft intimal hyperplasia, as chemopreventive against colon carcinogenesis, prostate and lung cancer.” * DMSO Related to MSM, but yet it is different enough to put in its own category. “One of the most well known and exotic properties of this solvent is its ability to penetrate living tissue and transport other medicines in their integral state deep into the body.” “We’ve barely scratched the surface [of DMSO’s capabilities], for this is a new principle in medicine. We’ve only had three new principles in our century – the antibiotic principle, the cortisone principle, and now the DMSO principle – and the DMSO principle is the only one of our generation. Despite all the controversy, my guess is that history will record it this way.” “At Mount Sinai Hospital in New York City, Charlotte Friend MD has turned cancerous cells into harmless, normal ones in the test tube by putting them in touch with the DMSO solutions. DMSO is routinely used by alternative cancer clinics in Mexico to transport laetrile intravenously into the body. Because of extremely promising clinical results, research is still ongoing on a privately funded basis into DMSO’s potential role in the breaking up of tumors and the killing of metastatic cancer cells in its own right. Yet the United States Food & Drug Administration and the UK Medicines Control Agency continue to forbid the advertising and retailing of DMSO for any medicinal purposes save one: for the treatment of the rare urinary bladder condition, interstitial cystitis.” Dries Cancer Diet The most essential food in this diet is the pineapple, known to be a valuable cancer treatment food. “The Dries cancer diet is based largely upon the consumption of raw fruits, mostly tropical fruit such as pineapple and mango, as well as certain raw vegetables, seeds and condiments

such as yoghurt, buttermilk and some oils. The basis of the selection of these foods is their bio­ energetic value measured in bio photons, which apparently have an effect upon resistance to cancer.” See the book: The Dries Cancer Diet: A Practical Guide to the Use of Fresh Fruit and Raw Vegetables in the Treatment of Cancer. “Based on the author’s research with over 300 cancer patients, this book explains the relationship between food, cancer and environmental factors.” * Echinacea (herb) “Echinacea stimulates the white blood cells that help fight infections in the body. Research has shown that echinacea enhances the activity of a particular type of white blood cells­macrophages. A particular glycoprotein in echinacea was found to significantly increase the killing effect of macrophages on tumor cells. A number of studies have found that echinacea boosts the body’s ability to fight Listeria, a bacterium that causes a deadly form of food poisoning, and Candida yeast.” Echinacea is actually a family of herbs. ** Ellagic Acid “Ellagic acid is a naturally occurring phytonutrient, chemically belonging to the phenol compound. It is a phenolic lactone compound found in a variety of fruits and vegetables. It occurs in particularly high concentrations in strawberries, raspberries, cranberries, grapes and many other berries, and in nuts such as walnuts and pecans (Daniel, E.M. et al., 1989). It is present in plants in the form of hydrozable tannins called ellagitannins. Ellagitannins are esters of glucose with hexahydroxydiphenic acid; when hydrolyzed, they yield ellagic acid in numerous plants such as those mentioned above.” Emanuel Revici (biologically guided chemotherapy) “Emanuel Revici, MD, treated many forms of cancer with great success for 40 years at his clinic in NY. The core of his therapy was a form ofselenium. He was driven out of practice by the New York Medical Board.” Escharotics A category of cancer salves used to treat external cancers. Several different herbs, and several different combinations of herbs and other ingredients have a highly successful history of treating skin cancer. ** Essiac Tea One of the absolutely most successful and most used of all alternative cancer treatments is an herbal product consisting of from 4 to 8 herbs, depending on who you ask. Rene Caisse was persecuted in Canada forcuring cancer patients. See my detailed article linked to on my main page * Exercise With Oxygen Therapy (EWOT) An excellent and safe way to get massive amounts of oxygen into your bloodstream. In a nutshell, you breathe an oxygen mixture while you walk on a treadmill. There is a book on the subject: Stop Aging or Slow the Process, How Exercise With Oxygen Therapy (EWOT) Can

Help, by William Campbell Douglass. Oxygen, of course, is the archenemy of cancer cells. There are dozens of different oxygen therapies in the alternative camp. Falk Supplementation Schedule This is a detailed supplement schedule that includes Vitamin C, niacinamide, Vitamin E, B­ Complex, etc. While there are no statistics that I know of for this treatment plan it appears to be a supercharged Hoffman plan. Its cure rate would clearly outperform orthodox treatments (as Hoffman’s did and as the weaker Pauling/Cameron plan did), but does not appear strong enough to be a stand­alone plan. Rather, it would be wise to have a base alternative treatment plan (e.g. laetrile), and then add the Falk supplements to the base plan. As long as you are at it, you might as well add the rest of the Rath Cellular Solution supplements. Seehttp://www.mnwelldir.org/docs/nutrition/diet.htm Flaxseed / Linseed Oil see Bugwig Fruitarian Diet As with the Raw Food diet, there is no universally agreed upon “Fruitarian Diet” for cancer. Rather, it is a diet based on fruits, nuts, seeds and berries (which are fruits). A diet based on a random selection of these items would be a weak cancer diet. However, this would be an excellent cancer diet on three conditions. First, eat only the fruits, nuts, seeds and berries which are known to treat cancer. Second, drink the juice of some of the key vegetables that treat cancer (e.g. carrots, cabbage, beatroot). Third, eat and drink nothing not just mentioned. See also the Raw Food diet. Fulvic Acid There are mixed reviews. Waiting for more information from the alternative camp who are not vendors. Gamma Linolenic Acid (GLA) (Borage oil) GLA is a “good” omega 6 fatty acid. ” In one study, terminally ill patients suffering from pancreatic cancer tripled their life expectancy after taking extensive doses of GLA. It is also believed that tumor growth and metastasis can be quelled with GLA­especially in melanoma and colon or breast cancer.” * GEIPE Cancer Therapy GEIPE is a direct current technology that has been around for awhile, but has not been pursued because it cannot be patented (e.g. it has no massive profit potential). It is concerned ONLY with shrinking tumors. “In the 1985 study, gentle electrotherapy was shown to consistently and progressively reduce the tumor mass. Five one­hour treatments over 5 days at 2.4 milli­ampere resulted in 98% reduction. 98% is not that far from 100%, or cure. In fact, 4 of the 6 animals in this group had no visually identifiable tumor remaining.” The website where this technology is being discussed is: See: http://www.cancer­treatment.net/

Genistein “Cancer patients whose cancer cells have intact functional p53 oncogene should *not* use genistein. Cancer cells that lack the p53 oncogene or have a mutated p53 oncogene have been shown to respond well to genistein. Only a pathological examination of cancer cells can determine the p53 status.” Geraniol “We show that geraniol, a monoterpene found in essential oils of fruits and herbs caused inhibition of Caco­2 cells growth and blocked cancer cell differentiation. Geraniol at 400 ìM prevented the formation of brush­border membranes and inhibited the expression of intestinal hydrolases (sucrase, lactase, alkaline phosphatase). When combined with geraniol (400 ìM), the antiproliferative and cytotoxic effects of 5­FU (5 ìM) were increased by 2­fold.” See: http://www.annieappleseedproject.org/geroil5fuhum.html * Germanium­132 “These T and B lymphocytes, macrophages and natural killer cells devour foreign cells and defend the body against viruses and cancer. However, none of these cells can function properly if the person is deficient in interferon, a protein which blocks viruses from infecting cells. Germanium appears to significantly enhance the body’s production of interferon. At the annual meeting of the Japan Cancer Association II in 1979, proceedings showed that germanium was a significant interferon­inducing agent. Studies published in the 1984 Journals of Interferon Research 4 confirmed these results.” Gerson Therapy Max Gerson was a doctor who created many types of pastes and products to treat cancer. There is currently a clinic that uses the Gerson Therapy. His book is called: A Cancer Therapy. Ginger Root “Researchers at the University of Minnesota determined that mice fed the main active component in ginger root three times a week had slower rates of cancer growth than control animals did.” Glandulars “A study involving calf thymus extract, published in the New England Journal of Medicine, is perhaps the most notable among glandular studies because it is the first controlled U.S. study to test glandulars on humans. Seventeen patients suffering from rare but fatal lymphocytic abnormalities involving tumorous growths and organ lesions (Letterer­Siwe disease, Hand­ Schüller­Christian disease and eosinophilic granuloma) were injected daily with thymus peptide extract. A control group of 20 people underwent chemotherapy, the normal treatment for these diseases. Ten of the 17 patients who responded to thymic extract experienced full remission after one year. Seven of the 17 showed either no change or clinical worsening after 28 days and therapy was discontinued. This remission rate was statistically comparable to previous study controls who were treated with chemotherapy.” I might add – without the side­affects! ** Glyconutrients

“Glyconutrients are a new and more specialized type of nutraceutical. They support the process our individual tissue cells use to recognize, and communicate with, each other. The work that Dr. Blobel [1999 Nobel Prize] and many others have done let us understand the importance of good cell communication to ensure good health. The discovery of Glyconutrients and their role in human health is now in Mainstream Medicine.” The potential for using glyconutrients in alternative cancer treatments is enormous. Glyconutrients are actually 8 specific monosaccharides. One of these 8 glyconutrients has 4 different forms. Of these 4 forms, one is the most important: the extremely rare long­chain acemannan, found mainly in aloe vera. At least 6 people (at last count) have been arrested, jailed or harassed by U.S. and Canadian authorities in an attempt to keep this molecule out of your home. A product called Albarin had a 94% cure rate of hospice cancer patients. The owner was arrested. I know of only one product that currently has this molecule: Aloe Immune. See my Stage IV cancer article for more information. * Grains – Whole Grains “Whole grains like oats, wheat, rye, millet and others contain fiber that helps isolate cancer causing compounds and removes them from the body. Flaxseed, rye and millet are rich in lignans which act as weak estrogens helping stymie the growth of breast cancer and other malignancies that are often estrogen dependent. The more whole grains you eat, the lower your odds of death, a University of Minnesota study suggests. Middle­aged women who ate slightly more than one whole­grain food per day had a 15% lower death rate than women eating lots of refined processed grains. That calls for more whole­grain dark bread and cereals such as All Bran and “old­ fashioned” oatmeal. Whole grains contain anti­cancer agents and help stabilize blood sugar and insulin, which may promote longevity.” * Grape Seed Extract + Grape Skin Extract Supplements that contain several cancer killing nutrients, such as resveratrol and OPCs. See Brandt Grape Cure. ** Graviola Tree / Brazilian Paw Paw There is fairly reliable evidence that a pharmaceutical company sat on this product for several years trying to synthesize one of its nutrients. In any case, there is very good scientific evidence from studies that this product is for real, if the quality of the manufacturing is good. See my detailed article linked to on my main page ** Green Tea Green Tea Polyphenols (GTP), particularly EGCG or EGCg (epigallocatechin gallate), not only inhibit an enzyme required for cancer cell growth, but also kills cancer cells with no ill effect on healthy cells. See my detailed article linked to on my main page Haelan 851 / Haelan 951 (add wheatgrass) Soy product

Hallelujah Acres A health retreat that specializes in wheatgrass juice and a largely raw food diet (though some cooked vegetables are allowed). As I have seen it described, it looks like an excellent cancer diet. The main criticism I have heard is how expensive it is. See http://www.nutritionhighway.com/hdiet.html Hans Nieper / Eumetabolic Treatment Harold Manner Cocktail (Metabolic) Herceptin Hoxey Treatment / Hoxey Formula ** Hulda Clark Therapies Dr. Clark has written several books on health subjects. She has a loyal following and there are a lot of testimonials about her on the internet. Her treatments include a number of herbs and nutrients. I do not doubt her treatments work well. Perhaps the most extreme of her treatments has to do with fixing a patient’s teeth and eliminating dental amalgam (mercury) and root canals (perpetual infections). While these things need to be dealt with, it personally seems extreme to me as part of a treatment plan (to me they belong in the long term prevention category). * Hydrazine Sulfate Hydrazine sulfate is another one of those treatments that has been persecuted by orthodox medicine. It is cheap to buy and more effective than orthodox treatments. It is not one of the best alternative treatments, but it is close to being in the “top 50” in my opinion. See my detailed article linked to on my main page ** Hydrogen Peroxide / H2O2 This is one of the oxygen therapy diets that is designed to get more oxygen to the cancer cells (cancer cells die if they get exposed to too much oxygen). It does this because Hydrogen Peroxide is really nothing but water with an extra oxygen molecule. It is one of the most used alternative treatments in the world. See my detailed article linked to on my main page * Hydroxygen Plus Hyperbaric Oxygen Therapy (HBOT) Hypercin see St. John’s Wort Hyperthermia Imm­Kine Immuno­Augmentative Therapy (IAT) “Another hotly contested and controversial treatment is Immuno­Augmentative­Therapy (IAT),

developed by another persecuted genius, Lawrence Burton, Ph.D, who has also been driven to [practice] outside the US to the Bahamas. Prior to yet another public debacle, Burton’s research had also been published in mainstream journals.” Immuno Placental Therapy (IPT) “Dr Valentine Govallo, a Russian immunologist … developed a vaccine from the human placenta after a live birth which appears to wake up the immune system of cancer patients to this evasion. Records of Govallo’s first trial with 45 patients and IPT in 1974 show 29 (64.4%) still alive after more than 20 years. Side effects are typical of many vaccinations: fever, malaise, flu­like symptoms for 1 to 2 days. Due to the strong possibility of “tumour lysis syndrome” because of the newly mounted immune attack, it is critical that detoxification and full nutritional strategies, including the use of coffee enemas, be in place before a course of IPT.” Immunotherapy see Coley’s Toxins Indirubin Induced Remission Therapy (IRT) (Dr. Sam Chachoua) ** Inositol / IP­6 / IP6 (inositol hexaphosphate) Insulin­induced Hypoglycemic Therapy (IHT) “BioPulse [in Mexico, of course] only began using IHT in June, 1999. At the time this article is being written, they have used this therapy on thirty­two patients, with about thirty treatments each. Every patient treated has had their tumors substantially reduced or completely eliminated … IHT involves intravenously introduced insulin to produces a state of profoundly lower blood sugar [cancer cells thrive on blood sugar]. This state lasts for a period of about an hour under careful clinical supervision. It is this hypoglycemic condition that changes the environment, in the body, to one in which cancer cells cannot survive.” Insulin Potentiation Therapy or Insulin Primed Potentiation Therapy (IPT) (Dr. Donato Perez Garcia) This is a therapy that uses purely orthodox substances (since orthodox medicine doesn’t use it, many people consider it an alternative treatment) that is far superior to other orthodox methods. It has far less side­effects because it uses far less chemotherapy. It targets cancer cells better than other orthodox treatments. Unfortunately, it is not as profitable as other treatments so it is ignored by orthodox medicine. The reason I do not push this treatment is that a person needs to go into an insulin coma before it is administered. I question the safety of doing this, and wonder why it is necessary. * Ionized / Alkaline Water Ionized water inhibits the spread of cancer and aids in killing cancer cells, directly or indirectly. However, there is not enough evidence to categorize it as a stand­alone treatment plan. See my detailed article linked to on my main page

Iscador see Mistletoe Isoprinosine Issels Treatment Jason Winters Tea Jiaogulan * Jon Barron Baseline of Health This is an excellent overall health book, with a separate chapter on cancer. His book, which is free and is online, covers everything from bowel cleanses to nutrition to how to buy vitamins. It is an excellent book, but should not be considered a solo cancer treatment. It should be considered mainly as a digestion, immune building and detox diet. See my detailed article linked to on my main page Josef Ostlund Diet This is a vegan type diet. ** Kelley Metabolic Program This is one of the most successful treatment plans ever, with a 93% cure rate on 33,000 patients, most of whom were declared terminal before they went to Kelley. However, because it is metabolic oriented, it can take several months to become fully effective. Kelley did not count in his statistics those who died within 12­18 months of first seeing him (I don’t know how many people fit into this category). The program can be found in the book: Cancer: Curing the Incurable Without Surgery, Chemotherapy or Radiation, by William Donald Kelley. Kelley was mercilessly persecuted by orthodox medicine and his treatment had to move to Mexico. See my detailed article linked to on my main page Kerosene Treatment This treatment is used in poorer countries because it is extremely cheap. Koch Treatment Kombucha (Mushroom) * Korean Red Ginseng “Korean Red Ginseng contains polyacetylene compounds that promote inhibiting effects on the growth of cancer cells by suppressing its mutation and metastasis, while promoting the activity of natural anti­cancer cells. Korean Red Ginseng’s’ health­promoting effects act on restoring the immune system debilitated by the cancer disease.” Krebiozen ** Laetrile – Amygdaline – Vitamin B17 – Nitrilosides One of the best known, and most proven, cancer treatment on earth. It has been severely

suppressed by our government, which has even reached into Mexico to stop this treatment! See my detailed article linked to on my main page Lapacho see Pau d’Arco * Licorice root (herb – Glycyrrhiza glabra) Licorice root contains both the isoflavone Licochalcone­A and triterpenoid saponin. Regarding Licochalcone­A, in a scientific study: “Cells from patients with leukemia, breast and prostate cancer that were grown in cultures in the lab, he says, were killed when he added enough of the extract.” Regarding triterpenoids, in a study: “They may block the production of prostaglandin – a hormone­like fatty acid that may be responsible for stimulating the growth of cancer cells – and help get rid of cancer­causing invaders. Triterpenoids have been shown in test tubes to stunt the growth of rapidly multiplying cells, like cancer cells, and they may even help precancerous cells return to normal.” As if that weren’t enough: “Medical researchers have isolated several active substances in licorice root including glycosides, flavonoids, asparagine, isoflavonoids, chalcones and coumarins. Primary of these is Glycyrrhetinic acid.” Licorice root is toxic, so much work need to be done on this herb. Limonene “In an article in the journal Anticancer Drugs, the growth inhibitory effects of limonene and other monoterpenes (including perillyl alcohol) on pancreatic carcinoma cells carrying a K­Ras mutation were examined. Limonene caused an approximately 50% growth reduction.” See: http://cancer.lef.org/pancreatic­3.html Lipaen Live Cell Therapy Livingston­Wheeler Therapy * Locoweed (Astragalus bisulcatus) High in selenium Lorraine Day Treatment Program Low Dose Naltrexone (LDN) Lycopene (phytonutrient) Macrobiotic Diet * Magnesium Peroxide (oxygen therapy) Magnesium Water Magnetic and Low Voltage Electrical Therapies Maitake (mushroom)

** Mangosteen Fruit “We found that antiproliferative effect of CME [crude methanolic extract] was associated with apoptosis on breast cancer cell line by determinations of morphological changes and oligonucleosomal DNA fragments. In addition, CME at various concentrations and incubation times were also found to inhibit ROS production. These investigations suggested that the methanolic extract from the pericarp [skin] of Garcinia mangostana had strong antiproliferation, potent antioxidation and induction of apoptosis. Thus, it indicates that this substance can show different activities and has potential for cancer chemoprevention which were dose dependent as well as exposure time dependent.” See my detailed article linked to on my main page * Maximin (activated clay silicate mineral) Mayapple (extremely toxic) Podophyllotoxin (podofilox) and its derivatives, which come from the mayapple, is used to create the chemotherapy drug Etoposide (which is sold by Bristol­Myers Squibb as VePesid, aka VP­16). Meditation and Visualization There are actually several varieties of this category of treatment. Mediterranean Diet Melatonin (hormone, antioxidant, anti­aging) “Most antioxidant nutrients have difficulty penetrating cell membranes. Melatonin, on the other hand, enters cells and subcellular compartments with ease which is crucial in protecting intracellular molecules from oxidative damage. An antioxidant molecule must have access to subcellular compartments (i.e. the mitochondria) in order to quench the hydroxyl radicals, considered by some to be the most damaging of all radicals.” “Few people realize that melatonin is a cancer­killing hormone that can enhance the human immune system, protect against the toxic side effects of chemotherapy, and radiation therapy, and improve wound healing after cancer surgery. Even fewer are aware of ongoing clinical trials in which melatonin is being used to help cancer patients better manage their disease symptoms, improve their quality of life, and even increase their survival rates. [Note: melatonin should not be used with certain kinds of cancer.] See:http://www.lef.org/magazine/mag2004/jan2004_report_melatonin_01.htm Mercury (Dental Amalgam) The 21 Century Clinic states, “In 25 years of practice we have never seen a cancer patient without toxic levels of mercury.” “Mercury is a deadly poison, which paralyzes the immune system. With a compromised immune system the cancer is now free to explode. By the time cancer is found it has been growing for many years. Detoxification: First we remove amalgam fillings and root canals, then we begin to flush the mercury out of the body. We monitor detoxification and the removal of mercury by collecting the urine and checking for mercury concentrations. As the mercury level drops, the immune system begins to improve. This allows the immune system to again begin attacking the cancer microbe.” “Note: At the last 28th Annual Cancer Conventions

one of the doctors mentioned that the filling should not be taken out until the person has started into remission. That the mercury within cells should be removed using products such as www.Concentrated Seaweed Extract Modiflan (40 X’s more powerful than seaweed) & www.ImuPlus which both removes heavy metals.” The subject of removing mercury from the body is a large topic. I should note that the American Dental Association is just as corrupt as the American Medical Association. Metabolic Diet see Kelley Metabolic Methylglyoxal (the lost Koch Glyoxylide?) * MGN­3 MGN­3 has been clinically shown to increase Tumor Necrosis Factor (TNF – a cytokine involved in activating the immune system), and increase the number of critical white blood cells: NK­Cells, T­Cells and B­Cells. In other words, MGN­3 works by building the immunity system. See my detailed article linked to on my main page Milk Thistle (silymarin) * Minerals I mention this subject because there have been some important discoveries about various minerals in the prevention and treatment of cancer. it is a general topic with evidence that justifies a great deal more research. Miso – Tamari – Shoyu * Mistletoe / Iscador Widely used in Europe with a moderate cure rate. Any treatment with mistletoe needs to be supplemented with other alternative treatments. Moerman’s Anti­Cancer Diet Most diet based alternative cancer treatments avoid citrus fruits. This diet is based on citrus fruits. In its day and in its location it was an excellent treatment. By today’s standards its cure rate is fairly mediocre. See my detailed article linked to on my main page Monoterpenes “Monoterpenes appear to act through multiple mechanisms in the prevention and chemotherapy of cancer. Several researchers are investigating these mechanisms and finding that, although the exact mechanism was not what they had assumed, the monoterpenes, limonene, and perillyl alcohol [and perillic acid and geraniol] have a profound antitumor activity on pancreatic cancer (Elson et al. 1994; Gelb et al. 1995; Crowell et al. 1996; Gould 1997; Bardon et al. 1998; Crowell 1999).” See: Limonene, Perillyl Alcohol and Geraniol See: http://cancer.lef.org/pancreatic­3.html

* MSM / Organic Sulphur “… it was discovered in the fifties that MSM tends to turn malignant tumors into benign tumors. And then the research stopped. Why? MSM is cheap (if purchased in bulk, even cheaper) and cannot be patented.” MSM makes cells permeable (which is known to aid in the treatment of cancer), strengthens collagen (which helps stop the spreading of cancer and thus may turn malignant tumors into benign tumors), helps maintain a proper pH (which cancer cells don’t like), and causes a host of other benefits for treating cancer, some direct and some indirect. While MSM has cured cancer by itself, on this website MSM is used as a Trojan Horse to get microbe killing substances inside the cancer cells. It is used in the Dirt Cheap Protocol and several individual treatments (e.g. MSM/Vitamin C – See left side­bar under Supplemental Treatments). This is an article where you can find the recommended vendor of MSM (though the vendor calls it “Organic Sulphur”). See the “What Causes Cancer” article (see left side­bar) to understand the importance of killing the microbes inside the cancer cells (it reverts cancer cells into normal cells). MSM is also used for psoriasis and many other health conditions. Other psoriasis protocols include UVA (Ultraviolet A – available at most pet stores) and UVB, which has stricter safety rules. Multi­Factorial Approach to Cancer (MFAC) Multi­Wave Oscillator (MWO) There has been a recent surge of interest in a Russian made electro­magnetic machine called the “Multi­Wave Oscillator.” George Lakhovsky, a Russian doctor, had a 98% success rate in treating fatal cancers over an 11­year period with his machine. Lakhovsky’s device was used in the U.S. until 1942 and in Europe for about another 15 years. It was ordered removed from the US hospitals that were using it shortly after Lakhovsky was hit by a car and died in 1942. A non­profit company is researching the use of a newer version of the machine. See: http://www.rhinoed.com/mwo_research.htm ** Myrrh “What makes it such an exciting player in the anti­cancer field is not only how well it kills cancer cells in general, but how it kills those that are resistant to other anti­cancer drugs. “The myrrh compound definitely appears to be unique in this way; it is working where other compounds have failed,” says Rafi. Myrrh is believed to work by inactivating a protein called Bcl­2, a natural factor that is overproduced by cancer cells, particularly in the breast and prostate.” Neem ** Noni Juice Noni juice scares some people away because it is usually sold by multi­level marketing. But don’t let the marketing fool you, this is a very serious cancer treatment. My article lists several complaints about the way it is usually put together, but even so, it is a serious treatment. See my detailed article linked to on my main page

Nucleic Acids (2LC1 and 2LCL1) (homeopathic) “The doctors who pioneered their use in cancer have found that they “re­balance” the weakened immune system, and there are documented cases of recovery which include many advanced cancers – metastatic liver and breast cancer, [leukemias] etc. However, because nucleic acids are usually present in infinitesimal quantities, and high doses are toxic, the principles of homeopathy have been applied to their orthomolecular use.” * Oil of Oregano ” Greek investigators, publishing in the Journal of Agriculture and Food Chemistry, determined that oil of wild oregano even destroyed human cancer cells. While spices may oxidize or destroy pathogens, amazingly, they also act as antioxidants for human cells, specifically for the fats of the human cell membranes.” See: http://www.colloidalsilversolutions.com/oreganoresearch.html Oleander Soup While the oleander plant is very poisonous and very toxic (and must be handled with latex gloves), if a solution of oleander is diluted enough it can be made safe to drink or made into a cream. In other words, a person can essentially make the cancer treatment Anvirzel at home. The directions for this can be found on a Yahoo group called: “Anvirzel” and on the following link. See: http://www.mnwelldir.org/docs/cancer1/altthrpy3.htm#Oleander Olive Leaf Extract Omega­3 Oils (Essential Oils) “Fatty acids from fish oils and fatty fish [e.g. omega­3] can destroy the power station – the mitochondria – in certain types of cancer cells, making the cells commit suicide.” Also, see Budwig. ** Omegasentials ” Omegasentials™, takes Budwig’s work a step further. Mix it with water and you’ll see that it is already water­soluble. The oils do not rise to the top. It contains all the co­factors that potentiate (make work better) the action of omega­3 fatty acids, and they add the antioxidants needed to help control the healing oxidative energy created by bringing healthy oils into the body (not to mention cleaning up dead cancer cells).” See: http://www.mnwelldir.org/docs/cancer1/budwig.htm Orthomolecular Medicine (e.g. high dose Vitamin C, etc.) ** Osiecki Cancer Technique Similar to the Rath Cellular Solution, but significantly more extensive. It actually attacks the spreading of cancer in several different ways. Surprisingly, it includes all of the elements of the Rath Cellular Solution. See my detailed article linked to on my main page Ostlund

Oxygen Therapy This is a category, not a specific treatment. Treatments in this category include Hydrogen Peroxide, Ozone Therapy, HBOT, EWOT, Budwig Flaxseed, etc. ** Ozone Therapy / O3 (Infusion Bottle) This is an oxygen therapy that requires a medical professional to administer, usually an N.D., depending on what state you live in. A pint or so of your blood is extracted into an infusion bottle. Ozone is applied to the blood and the ozone kills the cancer cells. Then the blood is put back into your body, carrying some extra oxygen molecules with it. See my detailed article linked to on my main page Paleolithic Diets Pancreatic Enzymes See Kelley Metabolic Papaya leaves * Pao Pereira “Pao pereira is a tree native to Brazil. It was Doctor Mirko Beljanski, a former researcher at the Pasteur Institute (France), who first discovered the therapeutic action of pao pereira. His in vitro laboratory tests demonstrated that pao pereira extract effectively suppressed the proliferation of HIV, herpes viruses, cancer, and leukemia cells. From these encouraging results, Doctor Beljanski concluded that pao pereira could be useful in the fight against Aids, herpes, and cancer.” ** Pau d’Arco / Lapacho / Taheebo Tea An excellent product with extremely high cancer killing potential. The one problem with Pau d’Arco is that it is extremely difficult to process and maintain its potentcy. If the product doesn’t work – change vendors. PC SPES A prostate cancer product, PC SPES is manufactured in China and includes several herbs and other nutrients. Unfortunately, it was also found to contain 3 manufactured drugs: diethylstilbestrol (DES), a synthetic hormone, which can cause blood clots, a blood thinner, and a third manufactured drug. PC­SPES was taken off the market and may still be off of the market. Some doctors think that it was the DES that was allowing so many prostate cancer patients to be cured. This brings up the issue: if DES is taken out of PC­SPEC will it still work? Pecta­Sol (a special pH­altered form of citrus pectin) Periactin (antihistamine) Perillyl Alcohol “In an article in Cancer Letters, perillyl alcohol was shown to reduce the growth of pancreatic tumors injected into hamsters to less than half that of controls. Moreover, 16% of pancreatic

tumors treated with perillyl alcohol completely regressed, whereas no control tumors regressed (Stark et al. 1995). Perillyl alcohol and perillic acid are metabolites of limonene. Limonene is only a weak inhibitor of the isoprenylation enzymes of Ras and other proteins, whereas perillyl alcohol and perillic acid are more potent inhibitors (Hardcastle et al. 1999).” See: http://cancer.lef.org/pancreatic­3.html Periwinkle (Catharanthus roseus) Madagascar plant originally used to derive Vincristine (Oncovin or VCR) chemotherapy drug. Phenergan Phycotene Phytonutrients / Phytochemicals (category of natural plant chemicals) Phytochemicals are special kinds of nutrients that are found in plants. There are thousands of phytochemicals that have not yet been identified, much less tested for cancer prevention or cancer treatment properties. In spite of that, it is clear that phytochemicals are the future of medicine and health. Phytochemicals have been found to have far better antioxidant properties than any vitamin. Some of them have amazing cancer treatment capabilities (e.g. those found in purple grape juice, the graviolatree, Noni juice, herbs, etc.). Unfortunately, most research into phytochemicals is controlled by the pharmaceutical industry, which has no interest in natural treatments for anything (their interest is in mutating natural substances). Thus, much of the information about phytochemicals is suppressed. Our government, which is also controlled by Big Pharma, is equally uninterested in natural substances. * Poly MVA / PolyMVA (Brain Cancer) (Merrill Garnett) “Poly­MVA is a uniquely formulated nutritional supplement containing a proprietary blend of Palladium and alpha­lipoic Acid (which we refer to as LAPd), Vitamins B1, B2 and B12, Formylmethionine, Acetyl Cystiene, and trace amounts of Molybdinum, Rhodium, and Ruthenium. It is designed to provide energy for the compromised body systems by changing the electrical potential of human cells, increasing the charge density of DNA within the cell.” See my detailed article linked to on my main page Pomegranate Oil Poultices Protomorphogens (PMGs) ** Protocel / Cantron / Cancell / Entelev This is an older treatment that has a long history of success. Because Protocel contains its own antioxidants there is a long list of other alternative treatments that cannot be used with Protocel. It is effective with brain cancer, especially if combined with Graviola. See my detailed article linked to on my main page

PSK An extract product from the mushroom Coriolus versicolor. Psychotherapy Radiofrequency of the Lung Tumor Ablation Radionics ** Rath Cellular Solution (Dr. Matthias Rath) This treatment stops the spreading of cancer. This same treatment is also used to prevent heart disease. See my detailed article linked to on my main page ** Raw Food Diet (Walker, et. al.) Unless you are terminally ill and only have a short time to live, this is one of the most successful cancer diets there are. It is essentially a vegetable and fruit juice vegan diet, with the exception that at least 80% of the food in the diet must be vegetables and fruits known to have powerful cancer­fighting nutrients. See my detailed article linked to on my main page Red Clover ((Trifolium pratense) “The isoflavones isolated from red clover have been studied for their effectiveness in treating some forms of cancer. It is thought that the isoflavones prevent the proliferation of cancer cells and that they may even destroy cancer cells. Laboratory and animal studies have found that red clover isoflavones may protect against the growth of breast cancer cells. This is surprising because estrogens (and isoflavones have estrogenic properties) have generally been thought to stimulate the growth of breast cancer in women. Until further research has been conducted and more information is available, the use of red clover isoflavones or other red clover products should probably be avoided in women with a history of breast cancer.” ” It is part of the famous Hoxey Formula for treating cancer, especially lymphatic cancer. Red clover ointment may be applied to lymphatic swellings.” “A 66­year­old physician with prostate cancer took a concentrated phyto­ estrogen based on red clover for just one week and thereby caused his tumour to regress. The patient had been diagnosed with a high PSA level (13.1 micrograms/liter) in March 1996 and a subsequent needle biopsy had confirmed the presence of a low grade adenocarcinoma. He was scheduled for a radical (suprapubic) prostatectomy and, on his own initiative, decided to take a daily dose of 160 mg of a phyto­estrogen product based on red clover (Promensil tablets – 4 X 40 mg/day) for the seven days preceding his operation. After the operation the biopsy tissue and the tumour tissue were compared. It was clear that the tumour tissue showed a high degree of apoptosis (cell death) resembling the effect of high­ dose estrogen therapy and consistent with tumour regression. Professor Stephens concludes that this case history provides further evidence that phyto­estrogens may prevent prostate cancer. He also points out that there were no adverse effects of the phyto­estrogen treatment.” Stephens, Frederick O., Medical Journal of Australia, Vol. 167, August 4, 1997, pp. 138­40

Reishi (Mushrooms) Resveratrol See Grape Skin Extract Retinoic Acid (Vitamin A derivative) Rhododendron Caucasicum Rhodiola Rosea Richard Schulze Treatment Rife Machine See Royal Rife Machine Robert / Bob Beck Silver Pulser Robert / Bob Beck Magnet Pulser Rooibos Tea * Royal Jelly (a bee product) [Canadian research team] “The criterion we used in these experiments was survival. The mice either developed leukaemia or tumours or were fully protected. Control mice died from ascitic tumours in less than 14 days, while mice receiving appropriate mixtures of cells and royal jelly all failed to develop tumours. Protected mice were kept under observation for 90 days after death of the control mice. They were then sacrificed and autopsied to confirm the absence of tumours.” Royal Rife Machine Royal Rife’s electromagnetism machine had a 100% cure rate of terminal cancer patients. This infuriated the AMA. The AMA persecuted Royal Rife to the extreme. His lab was burned down, one of his associates was murdered, his equipment was hunted down and destroyed, Rife himself may have been murdered, etc. A group in England claimed to obtain a Royal Rife machine that was not destroyed. A website which discussed old versions of the Royal Rife machine and other versions of it, and has a copy of the English report can be found at this web site. See: http://www.scoon.co.uk/ Also see this web site (this is a vendor and a source of superb information): See: http://www.rifecranerockwell.com/ Where to Buy the ICRF “Research Model” High RF Frequency Generator and Plasma Tube Amplifier: http://www.FrequencyStore.com Copy and paste the above website address into your web browser.Go to the vendor’s website that sells the High RF Frequency Generator and oscillator amplifier which can broadcast high RF frequencies using a plasma ray tube antenna. You’ll be able to see photographs of the generator and amplifiers including full specifications and pricing at their

website. Scrophularia (both a product and figwart) (Dr. Eli G. Jones) * Seabuckthorn oil Sea Salt – Unrefined Seasilver (trace minerals) Seaweed, Kelp * Selenium “It’s time to stop ignoring the facts?” say Richard A Passwater, PH.D., in his book, Selenium as Food & Medicine. “If you want to maintain your good health, increase your resistance to disease, and assure a long and energetic life, it is vitally important that you increase your intake of selenium.” Selenium protects the membranes of each of our body’s 60 trillion cells. The more selenium consumed the lower the incidence of cancer. . . . several physicians have found that when sufficient selenium is ingested by cancer patients to raise their blood levels of selenium to the desired range, their tumors began to shrink” Dr. Gerhard Schrauzer of the University of California says: “If every woman in America started taking selenium today, or had a high­selenium diet, within a few years the breast cancer rate in this country would drastically decline.” Dr. Raymond Shamberger of the Cleveland Clinic Foundation advises people to increase their intake of selenium to 200 micrograms a day, because “it can reduce the cancer rate dramatically for some type of cancers, particularly cancer of the colon, breast, esophagus, tongue, stomach, intestine, rectum, and bladder.” See: http://stopcancer.com/deathbed1.htm Sesbania Shackleton Grape Cure Shackleton had cancer and read the book by Johanna Brandt. He developed his own version of the diet, cured his cancer and wrote a book. I have not seen the book. * Shark Cartilage * Shark Liver Oil – Alkylglycerols * Sheep Sorrel (Herb) “Sorrel contains a high amount of nutrients including chlorophyll. Chlorophyll closely resembles hemoglobin, the red pigment in human blood, but has at its center a magnesium atom, whereas hemoglobin has an iron atom, and both carry oxygen to every cell of the organism. The chlorophyll molecules that carry oxygen through the bloodstream may do the following: inhibit chromosome damage to effectively block cancer, reduce the damage of radiation burns, kill germs and prevent the growth of harmful bacteria, strengthen the cell walls which may; improve the vascular system, heart function, intestines, lungs and uterus, aid in the removal of foreign deposits

from the walls of blood vessels, remove inflammation of the pancreas, purify the liver and increase the body’s ability to utilize oxygen by raising the oxygen level in the tissue cells.” See also Essiac Tea Silibinin (plant) “The silibinin­treated [cancer] cells that are unable to grow follow a differentiation pathway as evidenced by neuroendocrine­like morphology, elevated prostate tissue differentiation markers… and altered cell­cycle regulatory molecules.” Sister Mary Eymard Poydock, Ph.D. A Vitamin C and Vitamin B­12 protocol (marcytamin). Snow Rose (Alpine Tea / Rhododendron caucasicum) ” Rhododendron caucasicum inhibits or abolishes the activity of the enzyme hyaluronidase, known to be an initiator of colon cancer.” * Spirulina “Researchers at the Osaka Institute of Public Health in Japan gave volunteers over forty years of age 50 mL of a spirulina extract and measured the activity in the blood of interferon gamma and natural killer cells. For one to two weeks following the participants’ ingestion of spirulina, the activity of these substances was found to increase, and this increased activity continued for twelve to twenty­four weeks.” Squalamine An orthodox drug/chemical derived from the liver of sharks. In other words, the natural version of the molecule is illegal (because it cannot be patented), but the mutated version is legal. St John’s Wort (ingredient: hypercin) “In experiments using mice, hypericin was shown to accumulate specifically in tumor tissue. When these hypericin­treated mice were irradiated, tumor growth was inhibited. Similar results have been found in human tumor cell lines. Hypericin was taken up by the tumor cells, rendering them more vulnerable to the killing effects of specific types of light. These results suggest that hypericin can be used as a phototherapy tool when treating cancer.” Hypercin might be useful as a transport agent to get cancer­killing nutrients into cancer cells. It is already being tested for use with orthodox Photodynamic Therapy (PDT). Stockholm Protocol See Coenzyme Q10 Sulforaphane (e.g. Broccoli Sprouts) Superoxide Dismutase (SOD) Orthodox treatment ** Sweet Wormwood (herb) “Professor Henry Lai and Assistant Professor Narendra Singh of the University of Washington

have been conducting in vitro experiments to determine the effectiveness of Artemisinin [the active ingredient in sweet wormwood] in fighting cancer. A study concerning their research published in the Journal Life Sciences described how the compound killed virtually all human breast cancer cells exposed to it within sixteen hours. According to Dr. Lai, “Not only does it appear to be effective, but it’s very selective.” He continued “it´s highly toxic to the cancer cells, but has a marginal impact on normal breast cells.” The UN and UNICEF refused to allow sweet wormwood to treat malaria, for which it is a cure, until it could be combined with a profitable pharmaceutical drug. Chalk up a several million more deaths to Big Pharma’s greed and government corruption. Since malaria is a parasite, this plant could give credibility to the theory of some that cancer is caused by a parasite. See: http://www.cancer­coverup.com/newsletter/06­2004/default.asp Tagamet (Cimetidine) An over­the­counter orthodox drug for treating acid indigestion has significant evidence for treating cancer. In fact, it is more effective than chemotherapy (of course chemotherapy is useless, but Tagamet is not). See: http://www.second­opinions.co.uk/cimetidine.html Taheebo Tea see Pau d’Arco ** Tetrahydrocannabinol (THC – medical marijuana) Yet another heavily FDA suppressed treatment. “The term medical marijuana took on dramatic new meaning last February, when researchers in Madrid announced they had destroyed incurable brain cancer tumors in rats by injecting them with THC, the active ingredient in cannabis. Most Americans don’t know anything about the Madrid discovery. Virtually no U.S. newspapers carried the story, which ran only once on the AP and UPI news wires, on Feb. 29. The ominous part: This isn’t the first time scientists have discovered that THC shrinks tumors. In 1974, researchers at the Medical College of Virginia had been funded by the National Institutes of Health to find evidence that marijuana damages the immune system. Instead, they found that THC slowed the growth of three kinds of cancer in mice — lung and breast cancer and a virus­induced leukemia. The government quickly shut down the Virginia study and all further cannabis/tumor research, according to Jack Herer, who reports on the events in his book, The Emperor Wears No Clothes. In 1976, President Gerald Ford put an end to all public cannabis research and granted exclusive research rights to major pharmaceutical companies, which set out — unsuccessfully — to develop synthetic forms of THC that would deliver all the medical benefits without the “high.” See the web site: http://www.ardpark.org/research/shrinktumors.htm See the web site:http://old.newhavenadvocate.com/articles/potshots.html Tian Xian liquid Thalidomide An orthodox treatment that has had excellent results treating advanced cancer patients. “Thalidomide slows the progression of prostate cancer, but has serious side effects.” The FDA

has teamed up with the vendor of this product (or should I say the vendor has hired the FDA?) to make it very difficult to obtain this medicine. Whether this is because it is too cheap of a cure (it is very expensive by the way) or because of its side­effects, I do not know. But it has been around for many years and still does not have a generic. Theanine (amino acid) This is an amino acid in green tea. “The IDA [idarubicin (IDA)­induced antitumor activity and toxicity] concentration in the tumors in the theanine plus IDA group increased to twice the level in the IDA alone group. Furthermore, the decrease in tumor weight caused by IDA at 1.0 mg/kg per day x4 days (at this dose IDA exhibits antitumor activity) was significantly amplified by theanine. The numbers of leukocyte and bone marrow cells decreased significantly on IDA injection. Theanine significantly reversed these changes.” Tocotrienol Complex (a class of Vitamin E compounds) “It is encouraging to know that the in vitro tests that document the anti­cancer effects of tamoxifen also show tocotrienols to have similar cell inhibitory properties. Compared to tamoxifen, however, tocotrienols are safe. Human studies have shown that daily doses of up to 240 mg of tocotrienols for 16 months produce no adverse effects. Further studies will determine whether humans who saturate their breast adipose tissue with tocotrienol from supplements will achieve a reduced incidence of breast cancer. (Please note that it is the palm­oil tocotrienols, and not rice­bran tocotrienols, that have primarily demonstrated these anti­cancer effects.)” See the web site:http://www.lef.org/magazine/mag2002/may2002_cover_vitamine_02.html ** Transfer Factor “In 1949, Dr. H. Sherwood Lawrence made a revolutionary discovery while studying tuberculosis. He determined that an immune response could be transferred from a donor to a recipient by injecting an extract of leukocytes. He found that this extract contained a factor capable of transferring immunity. He named the substance transfer factor. In the fifty years since Lawrence’s pioneering work, an estimated $40,000,000 has been spent on research, resulting in over 3,000 scientific papers documenting the benefits of transfer factors. The world’s leading scientists and physicians have established the safety and remarkable immune system benefits of transfer factor. The processing methods that allow for large scale extraction of transfer factor have only recently been perfected and a commercial product has only been available within the last few years.” Triphala Triptolide TVZ­7 Lymphocyte Treatment “TVZ­7 is an extract of cytokines – immune system hormones – and other immune activating chemicals, taken from cultured B lymphocytes. Mature B lymphocytes are white cells that live in the spleen, lymph glands and peripheral lymphoid tissue. They can independently [recognize] “antigens”, anything foreign to the body, and mount an attack by creating antibodies. Whilst the scientists behind TVZ­7 are not yet quite sure how exactly it works, it has induced dramatic

responses in cases of pancreatic, liver and brain cancer, as well as having an impact on pain control.” Ukrain “Ukrain appears to halt cancer growth by interfering with oxygen respiration in cancer cells. Success rates with Ukrain in cancer are best in the early stages: 93% and 72% in Stages I and II, and 30% for advanced metastatic cancer. Laboratory testing to date indicates Ukrain can affect even cancers such as brain, lung and melanoma. This information comes from 10 year studies at the Ukrainian Anti­Cancer Institute in Vienna.” Umeboshi Ultraviolet Urea / Urine Yes, it is what you are thinking, but there is good science behind it. Ursodeoxycholic Acid (UDCA) “Colonic tumorigenesis involves the processes of initiation and promotion/progression from normal epithelial cells to tumors. Studies in both humans and experimental models of colon cancer indicate that secondary bile acids promote tumor development. In contrast, we have demonstrated previously that another bile acid, ursodeoxycholic acid (UDCA), inhibits the development of azoxymethane (AOM)­induced colon cancer in rats. More recently, we have shown that UDCA inhibits AOM­induced hyperproliferation, and aberrant crypt formation and growth.” Velázquez’s Approach to Cancer Vanadium “In one study done at the Parker Hughes Institute the minerals vanadium and sulfur were used to create 24 new drugs in the war against cancer. Vanadium was found to kill 14 different cancer cell lines both in the laboratory and in human cell lines.” VG­1000 (Govallo’s Vaccine) see Immuno Placental Therapy Vincrstine “He experienced several health challenges in the past decade (i.e., open­heart surgery, prostate cancer, and, most recently, multiple myeloma). Coincidently, the drug that until recently helped treat the myeloma was vincristine, a purified alkaloid derived from the Madagascar periwinkle (Catharanthus roseus (L.) G. Don, Apocynaceae). Vincristine is manufactured by the Eli Lilly company of Indianapolis, the company that funded Tyler’s chair at Purdue. Further, the drug was discovered in a classic scenario of serendipity by Tyler’s friend and colleague, the late Gordon Svoboda, who, while researching the periwinkle for its traditionally reputed antidiabetic activity, discovered its potential for treating certain cancers. Tyler had intended to write the story of Svoboda and vincrstine, as he considered it one of the great untold stories in pharmacognosy and drug discovery from plants.”

Vitamin C Therapy see Orthomolecular Medicine Vitamin D Analogue Therapy “Vitamin D given in large doses has been shown in laboratory work to make cancer cells “differentiate”, that is develop from the primitive forms characteristic of cancer cells into the more complex, [organized] forms of normal cells … at present, it must be considered solely adjuvant and not a sole therapy. Water Cure (Dr. F. Batmanghelidj) ** Wheat grass / Wheatgrass / Barley grass Ann Wigmore is probably the founder of this treatment plan. She died at the age of 95 in a fire. Her treatment is usually combined with other alternative treatments. See my detailed article linked to on my main page Wiedbrauck Treatment Willard’s Water ** Wormwood (herbal) “Research professor Henry Lai and assistant research professor Narendra Singh have exploited the chemical properties of a wormwood derivative to target breast cancer cells with surprisingly effective results. A study in the latest issue of the journal Life Sciences describes how the derivative killed virtually all human breast cancer cells exposed to it within 16 hours.” Yucca glaucoma “The active material found in our studies is sensitive to light and heat. Only the fresh yucca flowers possess anti­cancer activity, not the seeds, leaves, fruits or roots. The activity is lost when the flowers wilt or dry.” Yuccalive This herbal product is claimed by its vendors to be a scientifically proven (in China) cure for bone cancer. Yumeiho Therapy

Upcoming Events Don’t miss The Microbiome Medicine Summit! Did you know that your body is filled with bacteria? According to research we are 50% bacteria! These trillions of organisms (known collectively as the microbiome) live all over our bodies and have a profound influence on our health. But what do you know about

your microbiome? Understanding your microbiome is vitally important to understanding how to live a healthy life! This is especially true for people suffering from chronic diseasesof the heart and digestive system, autoimmune disease, diabetes, thyroid disorders and more.

The Microbiome Medicine Summit will teach you about: Strengthening your immune system Reducing inflammation Impacting genetic expression Improving metabolism Controlling caloric absorption Guiding your brain And more! Register (for FREE) today and you’ll get the following gifts: Expert Talk #1: Healing the Skin with Essential Oils by Dr. Eric Zielinski Expert Talk #2: Macro Implications of the Microbiome by Sayer Ji The Microbiome Medicine Summit is online and free from February 29­March 7, 2016