Experimental Biochemistry: A Student Companion 9789389633924

Table of contents :
Cover
Half Title
Title
Copyright
Preface
Acknowledgements
Contents
Chapter 1: Laboratory Safety and Hygiene
Chemical hazards
Biological hazards
Physical hazards
Electrical & Mechanical hazards
Standard operating procedures (SOP) for laboratory safety
Hazard management in the laboratory
Hazard symbols
Laboratory waste disposal
Chapter 2: Units and Measurements
Mass
Volume
Concentration of analyte expressed per unit volume
Concentration of analyte or solution expressed as percent
Density & specific gravity
Ionic strength
Chapter 3: Basic Statistical Concepts for Biochemical Analysis
Precision
Accuracy
Computation of mean, standard deviation, coefficient of variation and standard error
Chapter 4: Basic Concepts and Use of Instruments in Biochemical Analysis
Photometry and spectrophotometry
Laboratory centrifuge
Electrophoresis
Chapter 5: pU and Buffers
pH. scale
pH meter
Buffers
Chapter 6: Qualitative Analysis
Amino acids and proteins
Carbohydrates
Lipids & steroids
Chapter 7: Quantitative Analysis
Titrimetric experiments
Spectrophotometric and photometric experiments
Chapter 8: Biochemical Separation Techniques
Chapter 9: Biochemical Preparations
Chapter 10: Enzyme Assays
Enzyme classification
Units of enzyme activity
Measurement of enzyme activity
Chapter 11: Clinical Biochemistry
Blood analysis
Clinical enzymology
Gastric secretion
Urine analysis
Chapter 12: Immunochemical Methods
Chapter 13: Food Analysis
Qualitative analysis of some common food adulterants
Quantitative analysis: Proximate analysis
Analysis of anti-nutritional factors
Appendix
A. Physical constants
B. Visible light absorption, filters and complementary hues
C. Boiling point & density of some organic solvents routinely used in the laboratory
D. Molar absorption coefficients of selected biochemicals
E. Molarity, normality, % concentration and specific gravity of some acids and alkalies
F. Preparation of acid and alkali (1 N) solutions
G. pH indicators for volumetric analysis
H. Standard buffers for pH meter calibration
I. pH values (approximate value) of frequently used 0.1 M solutions
J. Amino acids: Abbreviations, molecular weight, isoelectric point & solubility
K. Molecular weight of certain proteins
L. Isoelectric point of few proteins
M. Determination of protein concentration by UV- absorption method
N. Saponification, iodine & acid values of some common oils & fats
O. Determination of nucleic acid concentration by UV- absorption method
P. Some normal human clinical chemistry values of blood, serum & urine
Q. Some useful web sites
Index
Backcover

Citation preview

Experimental Biochemistry

Experimental

A Student Companion

This book meets the requirements of B.Sc., B.Sc. (Hons), B.Sc. (Pharmacy), B.Tech. (Biotechnology), MBBS, B.Sc. (Agr.) and M.Sc. courses having practical biochemistry module. Beedu Sashidhar Rao received his Master’s degree in Biochemistry from the University of Hyderabad in 1981 and Ph.D. from Osmania University, Hyderabad, pursuing doctoral research work at the National Institute of Nutrition, in 1987. He joined the Department of Biochemistry, Osmania University, as a faculty member in 1988. Presently, he is working as an Associate Professor in Biochemistry. During 2002-04, he also served as the Coordinator & Chairman, BOS, of the Forensic Science Unit of the department. He is actively engaged in research and teaching postgraduate students over the past sixteen years. His research interests are in the areas of Food toxicology (Mycotoxins, Alkaloids and Pesticides), Biomarkers and Immunodiagnostics. He has authored several research papers, published in both national and international journals.

978-93-89633-92-4

0.35 0.3 0.25 0.2 0.15 0.1 0.05 0 –0.05

0

20

40

60

80

0

10

0

12

0

14

0

16

Fractrion number

Beedu Sashidhar Rao Vijay Deshpande

Beedu Sashidhar Rao Vijay Deshpande

Vijay Deshpande obtained his Master’s and doctoral degrees in Biochemistry from Osmania University, Hyderabad. He joined the Department of Biochemistry in 1984 as an Assistant Professor and became Professor in 1998. He also served as Head (1999-2001) and Chairman (2001-2003), BOS in Biochemistry, Department of Biochemistry, Osmania University. In addition to teaching, Prof. Deshpande has thirty years of research experience in the area of protein chemistry (growth hormone, prolactin, thyroglobulin of mammalian origin and ferritins of animal and plant origin). Several research papers authored by him have been published in both national and international journals.

A Student Companion Absorbance at 280 nm

The experimental format includes theoretical considerations, methodological aspects and their applications in practical exercises.

Biochemistry

Experimental Biochemistry

Experimental Biochemistry: A Student Companion is a practical laboratory manual for students pursuing biochemistry as a subject module at various universities. This book covers a wide variety of experiments in the area of qualitative and quantitative biochemistry, enzymology, biochemical techniques and preparations, clinical biochemistry, immunological methods and food biochemistry. Due emphasis has been given to laboratory safety and hygiene.

TM

` 455/-

Distributed by: 9 789389 633924

TM

©Copyright 2020 I.K. International Pvt. Ltd., New Delhi-110002. This book may not be duplicated in any way without the express written consent of the publisher, except in the form of brief excerpts or quotations for the purposes of review. The information contained herein is for the personal use of the reader and may not be incorporated in any commercial programs, other books, databases, or any kind of software without written consent of the publisher. Making copies of this book or any portion for any purpose other than your own is a violation of copyright laws. Limits of Liability/disclaimer of Warranty: The author and publisher have used their best efforts in preparing this book. The author make no representation or warranties with respect to the accuracy or completeness of the contents of this book, and specifically disclaim any implied warranties of merchantability or fitness of any particular purpose. There are no warranties which extend beyond the descriptions contained in this paragraph. No warranty may be created or extended by sales representatives or written sales materials. The accuracy and completeness of the information provided herein and the opinions stated herein are not guaranteed or warranted to produce any particulars results, and the advice and strategies contained herein may not be suitable for every individual. Neither Dreamtech Press nor author shall be liable for any loss of profit or any other commercial damages, including but not limited to special, incidental, consequential, or other damages. Trademarks: All brand names and product names used in this book are trademarks, registered trademarks, or trade names of their respective holders. Dreamtech Press is not associated with any product or vendor mentioned in this book. ISBN: 978-93-89633-92-4 EISBN: 978-93-89976-92-2 Edition: 2020

Experimental Biochemistry

Experimental

A Student Companion

This book meets the requirements of B.Sc., B.Sc. (Hons), B.Sc. (Pharmacy), B.Tech. (Biotechnology), MBBS, B.Sc. (Agr.) and M.Sc. courses having practical biochemistry module. Beedu Sashidhar Rao received his Master’s degree in Biochemistry from the University of Hyderabad in 1981 and Ph.D. from Osmania University, Hyderabad, pursuing doctoral research work at the National Institute of Nutrition, in 1987. He joined the Department of Biochemistry, Osmania University, as a faculty member in 1988. Presently, he is working as an Associate Professor in Biochemistry. During 2002-04, he also served as the Coordinator & Chairman, BOS, of the Forensic Science Unit of the department. He is actively engaged in research and teaching postgraduate students over the past sixteen years. His research interests are in the areas of Food toxicology (Mycotoxins, Alkaloids and Pesticides), Biomarkers and Immunodiagnostics. He has authored several research papers, published in both national and international journals.

978-93-89633-92-4

0.35 0.3 0.25 0.2 0.15 0.1 0.05 0 –0.05

0

20

40

60

80

0

10

0

12

0

14

0

16

Fractrion number

Beedu Sashidhar Rao Vijay Deshpande

Beedu Sashidhar Rao Vijay Deshpande

Vijay Deshpande obtained his Master’s and doctoral degrees in Biochemistry from Osmania University, Hyderabad. He joined the Department of Biochemistry in 1984 as an Assistant Professor and became Professor in 1998. He also served as Head (1999-2001) and Chairman (2001-2003), BOS in Biochemistry, Department of Biochemistry, Osmania University. In addition to teaching, Prof. Deshpande has thirty years of research experience in the area of protein chemistry (growth hormone, prolactin, thyroglobulin of mammalian origin and ferritins of animal and plant origin). Several research papers authored by him have been published in both national and international journals.

A Student Companion Absorbance at 280 nm

The experimental format includes theoretical considerations, methodological aspects and their applications in practical exercises.

Biochemistry

Experimental Biochemistry

Experimental Biochemistry: A Student Companion is a practical laboratory manual for students pursuing biochemistry as a subject module at various universities. This book covers a wide variety of experiments in the area of qualitative and quantitative biochemistry, enzymology, biochemical techniques and preparations, clinical biochemistry, immunological methods and food biochemistry. Due emphasis has been given to laboratory safety and hygiene.

TM

Distributed by: 9 789389 633924

TM