A study of the membranes of the Melanoplus differentialis (Thomas) egg with special reference to the isolation and some characteristics of the hatching enzyme

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A study of the membranes of the Melanoplus differentialis (Thomas) egg with special reference to the isolation and some characteristics of the hatching enzyme

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ft ST U B X Off I R S MEMBRAH£ 3 Off TUB HE&AB0 P L U 8 O I W a R S U f l A U S

(TH O M A S)

BOO.

W ITH S P S O I f t l, R B F SR SN C K

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& m am ® o h a r a c t s r i s t i o s

O f THB B a T C H IK Q KNZXM®

la m e s

H ervey

S h u tts

A d i s s e r t a t i o n s u b m itte d in p a r t i a l f u l f i l l m e n t o f t h e r e q u ir e m e n ts f o r t h e d e g r e e o f D o c to r o f P h ilo s o p h y , i n t h e D e p a r tm e n t o f Z o o lo g y , i n t h e G r a d u a te C o lle g e o f th e S t a t e U n iv e r s it y o f Iow a

J u n e , 1950

ProQuest Number: 10583789

All rights reserved INFORMATION TO ALL USERS The quality o f this reproduction is d e p e n d e n t upon th e quality o f th e c o p y subm itted. In th e unlikely e v en t that th e author did not send a c o m p le te manuscript and there are missing p a g e s, th e se will b e noted. Also, if material had to b e rem o v ed , a n o te will indicate th e deletion.

uest ProQuest 10583789 Published by ProQuest LLC (2017). Copyright of th e Dissertation is held by th e Author. All rights reserved. This work is p rotected against unauthorized copying under Title 17, United States C o d e Microform Edition © ProQuest LLC. ProQuest LLC. 789 East Eisenhower Parkway P.O. Box 1346 Ann Arbor, Ml 48106 - 1346

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TABLS OF FIGURES F ig u re I

P i e c e o f 15 1$ d a y s p o s t - d i a p a u s e o u t I cl© b e f o r e t r i a l d i g e s t i o n {c o n t r o l )

F ig ure 2

P i e c e o f 15 * 16 d a y s p o s t d i a p a u s e c u t i c l e a f t e r 48 h o u r s o f d i g e s t i o n I n p i e u r o p o d i a s o l u t i o n mad© from 11 d a y s p o s t d i a p a u s e e m b r y o s

F ig u re 5

P iece o f 15 - 1 6 d a y s p o s t d i a p a u s e

F ig u re 4

O ro ss s e c t i o n o f 14 d a y s p r e d i a p a u s e ©gg w h i c h h a s b e e n k e p t a t 25 C*

F ig u re 5

C r o s s s e c t i o n o f . 16 d a y s p r e d i a p a u s e e g g k e p t a t 1 7 . 6°0*

F ig u re 6

C r o s s © e e t i o n o f 18 d a y s p r e d i & p - a u s e ?: k e p t a t 17*8°C#

F igu re 7

Summary o f Some o f t h e E m b r y o lo g y o f M e l a n o p l u s d i f f e r e n t i a l ! s (Thom as)

F ig u re 8

Summary o f Some o f th© .Embryology o f M e l a n o p l u s d i f f e r e n t ! a l l s (T h o m as) F ost& lapaus©

c u t i c l e a f t e r 48 h o u r s o f d i g e s t i o n i n p i e u r o p o d i a s o l u t i o n mad© f r o m 1 5 d a y s p o s t d i a p a u s e e m b ry o s

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R e s u l t s o f P r o t e i n and C a rb o h y d ra te f e a t s on P l e u r o p o d i a l and E x tr a s $ a b r y o n i e S o l u t i o n s b e f o r e and a f t e r t r i a l D ig estio n 16 - I t

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SumtBarj o f t h i c k n e s s i n M i c r o n s o f W hite C u t i c l e s d u r i n g t r i a l D ig estio n

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R e s u l t s o f G r a s s h o p p e r ISgge o n ig a r t-latee

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D evelopm ent o f W h ite C u t i c l e t h i c k n e s s a t D i f f e r e n t t e m p e r a t u r e s 37 *

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E ^ V a l u e s a n d 5hfi*s o f t h e D e v e l o p i n g . W hite O u t ! c l e a r "

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vi

TABLE OF GRAPHS

Graph 1.

G r a p h 2*

The e f f e c t s o f t h e p r e p a r e d p l e u r o p o d i a l and extra-*em bryonl c s o l u t i o n s upon th e w h ite c u t i c l e s o f ot h e r eggs

25

The mean t h i c k n e s s o f t h e w h i t e c u t i c l e a i n eggs w hich d e v e lo p a t fo u r d if f e r e n t tem p eratu res d u rin g ’ pre-diapause

41

1

INTRODUCTION E x t e n s i v e r e s e a r c h h a s b e e n c a r r i e d o u t on t h e o r i g i n and t h e p h y s i c a l and c h e m ic a l p r o p e r t i e s t h e m e m b ra n e s s u r r o u n d i n g , t h e M elan o plus d i f t e r e n t i . a l l s

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